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dc.contributor.authorBhattacharyya, S.
dc.contributor.authorLiu, H.
dc.contributor.authorZhang, Z.
dc.contributor.authorJam, M.
dc.contributor.authorDudeja, P.K.
dc.contributor.authorMichel, G.
dc.contributor.authorLinhardt, Robert J.
dc.contributor.authorTobacman, J.
dc.date2010
dc.date.accessioned2022-06-23T04:08:42Z
dc.date.available2022-06-23T04:08:42Z
dc.date.issued2010
dc.identifier.citationCarrageenan-induced increase in interleukin-8 secretion is modified by pre-treatment with galactosidases and carrageenases, S. Bhattacharyya, H. Liu, Z. Zhang, M. Jam, P. K. Dudeja, G. Michel, R. J. Linhardt, J. Tobacman, Journal of Nutritional Biochemistry, 21, 906-913, 2010.
dc.identifier.urihttps://hdl.handle.net/20.500.13015/5250
dc.identifier.urihttps://doi.org/10.1016%2Fj.jnutbio.2009.07.002
dc.descriptionJournal of Nutritional Biochemistry, 21, 906-913
dc.descriptionNote : if this item contains full text it may be a preprint, author manuscript, or a Gold OA copy that permits redistribution with a license such as CC BY. The final version is available through the publisher’s platform.
dc.description.abstractThe common food additive carrageenan predictably induces intestinal inflammation in animal models. Mechanisms of carrageenan-induced NFκB and IL-8 stimulation include an immune-mediated pathway involving toll-like receptor 4 (TLR4) and B-cell lymphoma/leukemia 10 (BCL10) and a reactive-oxygen species (ROS)-mediated pathway. To determine how the structure of CGN contributes to its initiation of inflammation by these two distinct mechanisms, we treated carrageenans with galactosidases and carrageenases, and determined the impact on IL-8 secretion and BCL10 production. Hydrolysis of carrageenan by the enzyme α-1→(3,6)-galactosidase significantly reduced the increases in IL-8 and BCL10, but other galactosidases tested, including α-1→6-, β-1→4-, and β-1→3,6-galactosidases had no effect. In contrast, specific κ- or ι-carrageenases, which hydrolyze the β-1,4-galactosidic bonds, produced increases in IL-8 and BCL10, attributable to increased exposure of the immunogenic α-1→3-galactosidic epitope of carrageenan to TLR4. These results were consistent with induction of the innate immune response by an interaction of TLR4 with the unusual α-D-Gal-(1→3)-D-Gal epitope that is present in carrageenan. Activation of the ROS-mediated pathway was unaffected by treatment of κ-CGN with either κ-CGNase (3 mg/L), α-1→(3,6)-galactosidase (20 mU/ml), or these enzymes in combination, indicating that the changes in IL-8 production were attributable to effects on the TLR4-BCL10-mediated innate immune pathway of induction of inflammation. These findings provide new information about the specificity of the carbohydrate-protein interaction between carrageenan and TLR4 and may help to devise treatments that modify the immune reactivity induced by carbohydrate antigens.
dc.languageen_US
dc.language.isoENG
dc.publisherElsevier
dc.relation.ispartofThe Linhardt Research Labs Online Collection
dc.relation.ispartofRensselaer Polytechnic Institute, Troy, NY
dc.relation.urihttps://harc.rpi.edu/
dc.subjectBiology
dc.subjectChemistry and chemical biology
dc.subjectChemical and biological engineering
dc.subjectBiomedical engineering
dc.titleCarrageenan-induced increase in interleukin-8 secretion is modified by pre-treatment with galactosidases and carrageenases
dc.typeArticle
dcterms.accessRightsA full text version is available in DSpace@RPI
dc.rights.holderIn Copyright : this Item is protected by copyright and/or related rights. You are free to use this Item in any way that is permitted by the copyright and related rights legislation that applies to your use. For other uses you need to obtain permission from the rights-holder(s). https://rightsstatements.org/page/InC/1.0/
dc.creator.identifierhttps://orcid.org/0000-0003-2219-5833
dc.relation.departmentThe Linhardt Research Labs.
dc.relation.departmentThe Shirley Ann Jackson, Ph.D. Center for Biotechnology and Interdisciplinary Studies (CBIS)


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