Biology; Chemistry and chemical biology; Chemical and biological engineering; Biomedical engineering
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High-resolution preparative separation of glycosaminoglycan oligosaccharides by polyacrylamide gel electrophoresis, T. N. Laremore, M. Ly, K. Solakyildirim, D.V. Zagorevski, R. J. Linhardt, Analytical Biochemistry, 401, 236–241, 2010.
Separation of milligram amounts of heparin oligosaccharides ranging in degree of polymerization from 4 to 32 is achieved within 6h using continuous elution polyacrylamide gel electrophoresis (CE-PAGE) on commercially available equipment. The purity and structural integrity of CE-PAGE-separated oligosaccharides are confirmed by strong anion exchange high-pressure liquid chromatography, electrospray ionization Fourier transform mass spectrometry, and two-dimensional nuclear magnetic resonance spectroscopy. The described method is straightforward and time-efficient, affording size-homogeneous oligosaccharides that can be used in sequencing, protein binding, and other structure-function relationship studies.;
Analytical Biochemistry, 401, 236–241; Note : if this item contains full text it may be a preprint, author manuscript, or a Gold OA copy that permits redistribution with a license such as CC BY. The final version is available through the publisher’s platform.
The Linhardt Research Labs.; The Shirley Ann Jackson, Ph.D. Center for Biotechnology and Interdisciplinary Studies (CBIS);
The Linhardt Research Labs Online Collection; Rensselaer Polytechnic Institute, Troy, NY; Analytical Biochemistry; https://harc.rpi.edu/;