Author
Wang, Z.; Zhang, Z.; McCallum, Scott A.; Linhardt, Robert J.
Other Contributors
Date Issued
2010
Subject
Biology; Chemistry and chemical biology; Chemical and biological engineering; Biomedical engineering
Degree
Terms of Use
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Full Citation
NMR quantification for monitoring heparosan K5 capsular polysaccharide production, Z. Wang, Z. Zhang, S. A. McCallum, R. J. Linhardt, Analytical Biochemistry, 398, 275–277, 2010.
Abstract
Traditional chromatographic quantification methods for heparosan produced from the Escherichia coli K5 strain rely on extensive purification requiring laborious sample preparation. These methods are time-consuming, often resulting in sample loss during purification, and thus might not accurately reflect the amount of heparosan in the original mixture. A simple, sensitive 1H nuclear magnetic resonance (NMR) quantification method that directly quantifies heparosan K5 polysaccharide present in E. coli fermentation supernatant is described.;
Description
Analytical Biochemistry, 398, 275–277; Note : if this item contains full text it may be a preprint, author manuscript, or a Gold OA copy that permits redistribution with a license such as CC BY. The final version is available through the publisher’s platform.
Department
The Linhardt Research Labs.; The Shirley Ann Jackson, Ph.D. Center for Biotechnology and Interdisciplinary Studies (CBIS);
Publisher
Elsevier
Relationships
The Linhardt Research Labs Online Collection; Rensselaer Polytechnic Institute, Troy, NY; https://harc.rpi.edu/;
Access
A full text version is available in DSpace@RPI;
