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    NMR quantification for monitoring heparosan K5 capsular polysaccharide production

    Author
    Wang, Z.; Zhang, Z.; McCallum, Scott A.; Linhardt, Robert J.
    ORCID
    https://orcid.org/0000-0003-2219-5833
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    NMR QUANTIFICATION FOR MONITORING HEPAROSAN K5 CAPSULAR.pdf (228.8Kb)
    Other Contributors
    Date Issued
    2010
    Subject
    Biology; Chemistry and chemical biology; Chemical and biological engineering; Biomedical engineering
    Degree
    Terms of Use
    In Copyright : this Item is protected by copyright and/or related rights. You are free to use this Item in any way that is permitted by the copyright and related rights legislation that applies to your use. For other uses you need to obtain permission from the rights-holder(s). https://rightsstatements.org/page/InC/1.0/;
    Full Citation
    NMR quantification for monitoring heparosan K5 capsular polysaccharide production, Z. Wang, Z. Zhang, S. A. McCallum, R. J. Linhardt, Analytical Biochemistry, 398, 275–277, 2010.
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    URI
    https://hdl.handle.net/20.500.13015/5260; https://doi.org/10.1016/j.ab.2009.12.005
    Abstract
    Traditional chromatographic quantification methods for heparosan produced from the Escherichia coli K5 strain rely on extensive purification requiring laborious sample preparation. These methods are time-consuming, often resulting in sample loss during purification, and thus might not accurately reflect the amount of heparosan in the original mixture. A simple, sensitive 1H nuclear magnetic resonance (NMR) quantification method that directly quantifies heparosan K5 polysaccharide present in E. coli fermentation supernatant is described.;
    Description
    Analytical Biochemistry, 398, 275–277; Note : if this item contains full text it may be a preprint, author manuscript, or a Gold OA copy that permits redistribution with a license such as CC BY. The final version is available through the publisher’s platform.
    Department
    The Linhardt Research Labs.; The Shirley Ann Jackson, Ph.D. Center for Biotechnology and Interdisciplinary Studies (CBIS);
    Publisher
    Elsevier
    Relationships
    The Linhardt Research Labs Online Collection; Rensselaer Polytechnic Institute, Troy, NY; https://harc.rpi.edu/;
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    A full text version is available in DSpace@RPI;
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