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dc.contributor.authorHigashi, Kyohei
dc.contributor.authorHosoyama, Saori
dc.contributor.authorOhno, Asami
dc.contributor.authorMasuko, Sayaka
dc.contributor.authorYang, Bo
dc.contributor.authorSterner, Eric
dc.contributor.authorWang, Zhenyu
dc.contributor.authorLinhardt, Robert J.
dc.contributor.authorToida, Toshihiko
dc.date2012
dc.date.accessioned2022-06-23T04:11:25Z
dc.date.available2022-06-23T04:11:25Z
dc.date.issued2012-01-15
dc.identifier.citationPhotochemical preparation of a novel low molecular weight heparin, K. Higashi, S. Hosoyama, A. Ohno, S. Masuko, B. Yang, E. Sterner, Z. Wang, R. J. Linhardt, T. Toida, Carbohydrate Polymers, 87, 1737-1743, 2012.
dc.identifier.issn1448617
dc.identifier.urihttps://hdl.handle.net/20.500.13015/5273
dc.identifier.urihttps://doi.org/10.1016/j.carbpol.2011.09.087
dc.descriptionCarbohydrate Polymers, 87, 1737-1743
dc.descriptionNote : if this item contains full text it may be a preprint, author manuscript, or a Gold OA copy that permits redistribution with a license such as CC BY. The final version is available through the publisher’s platform.
dc.description.abstractCommercial low molecular weight heparins (LMWHs) are prepared by several methods including peroxidative cleavage, nitrous acid cleavage, chemical ß-elimination, and enzymatic β-elimination. The disadvantages of these methods are that strong reaction conditions or harsh chemicals are used and these can result in decomposition or modification of saccharide units within the polysaccharide backbone. These side-reactions reduce product quality and yield. Here we show the partial photolysis of unfractionated heparin can be performed in distillated water using titanium dioxide (TiO2). TiO2 is a catalyst that can be easily removed by centrifugation or filtration after the photochemical reaction takes place, resulting in highly pure products. The anticoagulant activity of photodegraded LMWH (pLMWH) is comparable to the most common commercially available LMWHs (i.e., Enoxaparin and Dalteparin). 1H NMR spectra obtained show that pLMWH maintains the same core structure as unfractionated heparin. This photochemical reaction was investigated using liquid chromatography/mass spectrometry (LC/MS) and unlike other processes commonly used to prepare LMWHs, photochemically preparation affords polysaccharide chains of reduced length having both odd and even of saccharide residues.
dc.description.sponsorshipNational Institutes of Health
dc.languageen_US
dc.language.isoENG
dc.publisherElsevier
dc.relation.ispartofThe Linhardt Research Labs Online Collection
dc.relation.ispartofRensselaer Polytechnic Institute, Troy, NY
dc.relation.ispartofCarbohydrate Polymers
dc.relation.urihttps://harc.rpi.edu/
dc.subjectBiology
dc.subjectChemistry and chemical biology
dc.subjectChemical and biological engineering
dc.subjectBiomedical engineering
dc.titlePhotochemical preparation of a novel low molecular weight heparin
dc.typeArticle
dcterms.accessRightsA full text version is available in DSpace@RPI
dcterms.isPartOfJournal
dcterms.isVersionOfhttps://doi.org/10.1016/j.carbpol.2011.09.087
dc.rights.holderIn Copyright : this Item is protected by copyright and/or related rights. You are free to use this Item in any way that is permitted by the copyright and related rights legislation that applies to your use. For other uses you need to obtain permission from the rights-holder(s). https://rightsstatements.org/page/InC/1.0/
dc.creator.identifierhttps://orcid.org/0000-0003-2219-5833
dc.relation.departmentThe Linhardt Research Labs.
dc.relation.departmentThe Shirley Ann Jackson, Ph.D. Center for Biotechnology and Interdisciplinary Studies (CBIS)
rpi.description.pages1737-1743
rpi.description.volume87


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