Author
Cai, Chao; Solakyildirim, Kemal; Yang, Bo; Beaudet, Julie M.; Weyers, Amanda; Linhardt, Robert J.; Zhang, Fuming
Other Contributors
Date Issued
2012-01-04
Subject
Biology; Chemistry and chemical biology; Chemical and biological engineering; Biomedical engineering
Degree
Terms of Use
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Full Citation
Semi-synthesis of chondroitin sulfate-E from chondroitin sulfate-A, C. Cai, K. Solakyildirim, B. Yang, J. M. Beaudet, A. Weyers, R. J. Linhardt, F. Zhang, Carbohydrate Polymers, 87, 822-829, 2012.
Abstract
Chondroitin sulfate-E (chondroitin-4, 6-disulfate) was prepared from chondroitin sulfate-A (chondroitin-4 - sulfate) by regioselective sulfonation, performed using trimethylamine sulfur trioxide in formamide under argon. The structure of semi-synthetic chondroitin sulfate-E was analyzed by PAGE, 1H NMR, 13C NMR, 2D NMR and disaccharide analysis and compared with natural chondroitin sulfate-E. Both semi-synthetic and natural chondroitin sulfate-E were each biotinylated and immobilized on BIAcore SA biochips and their interactions with fibroblast growth factors displayed very similar binding kinetics and binding affinities. The current semi-synthesis offers an economical approach for the preparation of the rare chondroitin sulfate-E from the readily available chondroitin sulfate-A.;
Description
Carbohydrate Polymers, 87, 822-829; Note : if this item contains full text it may be a preprint, author manuscript, or a Gold OA copy that permits redistribution with a license such as CC BY. The final version is available through the publisher’s platform.
Department
The Linhardt Research Labs.; The Shirley Ann Jackson, Ph.D. Center for Biotechnology and Interdisciplinary Studies (CBIS);
Publisher
Elsevier
Relationships
The Linhardt Research Labs Online Collection; Rensselaer Polytechnic Institute, Troy, NY; Carbohydrate Polymers; https://harc.rpi.edu/;
Access
A full text version is available in DSpace@RPI;
