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    Electron detachment dissociation and infrared multiphoton dissociation of heparin tetrasaccharides

    Author
    Leach, Franklin E.; Xiao, Zhongping; Laremore, Tatiana N.; Linhardt, Robert J.; Amster, I. Jonathan
    ORCID
    https://orcid.org/0000-0003-2219-5833
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    ELECTRON DETACHMENT DISSOCIATION AND INFRARED.pdf (1.204Mb)
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    Date Issued
    2011-12-01
    Subject
    Biology; Chemistry and chemical biology; Chemical and biological engineering; Biomedical engineering
    Degree
    Terms of Use
    In Copyright : this Item is protected by copyright and/or related rights. You are free to use this Item in any way that is permitted by the copyright and related rights legislation that applies to your use. For other uses you need to obtain permission from the rights-holder(s). https://rightsstatements.org/page/InC/1.0/;
    Full Citation
    Electron detachment dissociation and infrared multiphoton dissociation of heparin tetrasaccharides, F. E. Leach, Z. Xiao, T. N. Laremore, R. J. Linhardt, I. J. Amster, International Journal of Mass Spectrometry, 308, 253-259, 2011.
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    URI
    https://hdl.handle.net/20.500.13015/5276; https://doi.org/10.1016/j.ijms.2011.08.029
    Abstract
    Heparin glycosaminoglycans (GAGs) present the most difficult glycoform for analytical characterization due to high levels of sulfation and structural heterogeneity. Recent contamination of the clinical heparin supply and subsequent fatalities has highlighted the need for sensitive methodologies of analysis. In the last decade, tandem mass spectrometry has been increasingly applied for the analysis of GAGs, but developments in the characterization of highly sulfated compounds have been minimal due to the low number of cross-ring cleavages generated by threshold ion activation by collisional induced dissociation (CID). In the current work, electron detachment dissociation (EDD) and infrared multiphoton dissociation (IRMPD) are applied to a series of heparin tetrasaccharides. With both activation methods, abundant glycosidic and cross-ring cleavages are observed. The concept of Ionized Sulfate Criteria (ISC) is presented as a succinct method for describing the charge state, degree of ionization and sodium/proton exchange in the precursor ion. These factors contribute to the propensity for useful fragmentation during MS/MS measurements. Precursors with ISC values of 0 are studied here, and shown to yield adequate structural information from ion activation by EDD or IRMPD.;
    Description
    International Journal of Mass Spectrometry, 308, 253-259; Note : if this item contains full text it may be a preprint, author manuscript, or a Gold OA copy that permits redistribution with a license such as CC BY. The final version is available through the publisher’s platform.
    Department
    The Linhardt Research Labs.; The Shirley Ann Jackson, Ph.D. Center for Biotechnology and Interdisciplinary Studies (CBIS);
    Publisher
    Elsevier
    Relationships
    The Linhardt Research Labs Online Collection; Rensselaer Polytechnic Institute, Troy, NY; International Journal of Mass Spectrometry; https://harc.rpi.edu/;
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    A full text version is available in DSpace@RPI;
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