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    Response surface optimization of the heparosan N-deacetylation in producing bioengineered heparin

    Author
    Wang, Zhenyu; Li, Jennifer; Cheong, Samantha; Bhaskar, Ujjwal; Akihiro, Onishi; Zhang, Fuming; Dordick, Jonathan S.; Linhardt, Robert J.
    ORCID
    https://orcid.org/0000-0003-2219-5833
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    RESPONSE SURFACE OPTIMIZATION OF THE HEPAROSAN N-DEACETYLATION.pdf (6.861Mb)
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    Date Issued
    2011-12-10
    Subject
    Biology; Chemistry and chemical biology; Chemical and biological engineering; Biomedical engineering
    Degree
    Terms of Use
    In Copyright : this Item is protected by copyright and/or related rights. You are free to use this Item in any way that is permitted by the copyright and related rights legislation that applies to your use. For other uses you need to obtain permission from the rights-holder(s). https://rightsstatements.org/page/InC/1.0/;
    Full Citation
    Response surface optimization of the heparosan N-deacetylation in producing bioengineered heparin, Z. Wang, J. Li, S. Cheong, U. Bhaskar, O. Akihiro, F. Zhang, J.S. Dordick, R.J. Linhardt, Journal of Biotechnology, 156, 188-196, 2011.
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    URI
    https://hdl.handle.net/20.500.13015/5280; https://doi.org/10.1016/j.jbiotec.2011.08.013
    Abstract
    The chemical step in the chemoenzymatic synthesis of bioengineered heparin has been examined and optimized statistically using a response surface methodology. A four factor, two level full factorial design experiment and a three factor Box-Behnken design were carried out. The goal was to establish a method to prepare N-sulfo, N-acetyl heparosan of the desired N-acetyl content, number average molecular weight, and in maximum yield by controlling the reactant concentrations, reaction time and reaction temperature. The response surface models obtained were used to predict the reaction conditions required to optimally prepare N-sulfo, N-acetyl heparosan from E. coli generated heparosan starting material of different molecular weights.;
    Description
    Journal of Biotechnology, 156, 188-196; Note : if this item contains full text it may be a preprint, author manuscript, or a Gold OA copy that permits redistribution with a license such as CC BY. The final version is available through the publisher’s platform.
    Department
    The Linhardt Research Labs.; The Shirley Ann Jackson, Ph.D. Center for Biotechnology and Interdisciplinary Studies (CBIS);
    Publisher
    Elsevier
    Relationships
    The Linhardt Research Labs Online Collection; Rensselaer Polytechnic Institute, Troy, NY; Journal of Biotechnology; https://harc.rpi.edu/;
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    A full text version is available in DSpace@RPI;
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