AuthorYang, B.; Weyers, A.; Baik, J.Y.; Sterner, E.; Sharfstein, S.; Mousa, S.A.; Zhang, F.; Dordick, J.S.; Linhardt, Robert J.
SubjectBiology; Chemistry and chemical biology; Chemical and biological engineering; Biomedical engineering
Full CitationUltraperformance ion-pairing liquid chromatography with on-line electrospray ion trap mass spectrometry for heparin disaccharide analysis, B. Yang, A. Weyers, J. Y. Baik, E. Sterner, S. Sharfstein, S. A. Mousa, F. Zhang, J. S. Dordick, R. J. Linhardt, Analytical Biochemistry, 415, 59–66, 2011.
AbstractA high resolution method for the separation and analysis of disaccharides prepared from heparin and heparan sulfate (HS) using heparin lyases is described. Ultraperformance liquid chromatography in a reverse-phase, ion-pairing mode efficiently separates eight heparin/HS disaccharides. The disaccharides can then be detected and quantified using electrospray ionization mass spectrometry. This method is particularly useful in the analysis of small amounts of biological samples, including cells, tissues and biological fluids, as it provides high sensitivity without being subject to interference from proteins, peptides and other sample impurities.;
DescriptionAnalytical Biochemistry, 415, 59–66; Note : if this item contains full text it may be a preprint, author manuscript, or a Gold OA copy that permits redistribution with a license such as CC BY. The final version is available through the publisher’s platform.
DepartmentThe Linhardt Research Labs.; The Shirley Ann Jackson, Ph.D. Center for Biotechnology and Interdisciplinary Studies (CBIS);
RelationshipsThe Linhardt Research Labs Online Collection; Rensselaer Polytechnic Institute, Troy, NY; https://harc.rpi.edu/;
AccessA full text version is available in DSpace@RPI;