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dc.contributor.authorHickey, A.M.
dc.contributor.authorBhaskar, U.
dc.contributor.authorLinhardt, Robert J.
dc.contributor.authorDordick, J.S.
dc.date2013
dc.date.accessioned2022-06-23T04:14:42Z
dc.date.available2022-06-23T04:14:42Z
dc.date.issued2013
dc.identifier.citationEffect of eliminase gene (elmA) deletion on heparosan production and shedding in Escherichia coli K5, A. M. Hickey, U. Bhaskar, R. J. Linhardt, J. S. Dordick, Journal of Biotechnology, 165, 175–177, 2013.
dc.identifier.urihttps://hdl.handle.net/20.500.13015/5294
dc.identifier.urihttps://doi.org/10.1016/j.jbiotec.2013.03.018
dc.descriptionJournal of Biotechnology, 165, 175–177
dc.descriptionNote : if this item contains full text it may be a preprint, author manuscript, or a Gold OA copy that permits redistribution with a license such as CC BY. The final version is available through the publisher’s platform.
dc.description.abstractEscherichia coli K5 produces heparosan and sheds it into the growth medium in a temperature dependent manner. The shedding is believed to be controlled, at least in part, by enzyme action on the cell-associated capsular polysaccharide, heparosan. One candidate enzyme in such shedding is eliminase. The eliminase gene (elmA) was deleted from the genome of E. coli K5 and its effect on secreted and cell-associated heparosan was investigated. Deletion of the eliminase gene resulted in a significant reduction in heparosan shedding into the medium and heparosan content in the capsule of the cells, indicating its pivotal role in heparosan synthesis and shedding by E. coli K5.
dc.languageen_US
dc.language.isoENG
dc.publisherElsevier
dc.relation.ispartofThe Linhardt Research Labs Online Collection
dc.relation.ispartofRensselaer Polytechnic Institute, Troy, NY
dc.relation.urihttps://harc.rpi.edu/
dc.subjectBiology
dc.subjectChemistry and chemical biology
dc.subjectChemical and biological engineering
dc.subjectBiomedical engineering
dc.titleEffect of eliminase gene (elmA) deletion on heparosan production and shedding in Escherichia coli K5
dc.typeArticle
dcterms.accessRightsA full text version is available in DSpace@RPI
dcterms.isVersionOfhttps://doi.org/10.1016/j.jbiotec.2013.03.018
dc.rights.holderIn Copyright : this Item is protected by copyright and/or related rights. You are free to use this Item in any way that is permitted by the copyright and related rights legislation that applies to your use. For other uses you need to obtain permission from the rights-holder(s). https://rightsstatements.org/page/InC/1.0/
dc.creator.identifierhttps://orcid.org/0000-0003-2219-5833
dc.relation.departmentThe Linhardt Research Labs.
dc.relation.departmentThe Shirley Ann Jackson, Ph.D. Center for Biotechnology and Interdisciplinary Studies (CBIS)


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