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    Molecular weight characterization for glycosaminoglycans with different degrees of sulfation by size exclusion chromatography

    Author
    Wang, Z.; Zhang, F.; Dordick, J.S.; Linhardt, Robert J.
    ORCID
    https://orcid.org/0000-0003-2219-5833
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    MOLECULAR MASS CHARACTERIZATION OF GLYCOSAMINOGLYCANS WITH.pdf (1.728Mb)
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    Date Issued
    2012
    Subject
    Biology; Chemistry and chemical biology; Chemical and biological engineering; Biomedical engineering
    Degree
    Terms of Use
    In Copyright : this Item is protected by copyright and/or related rights. You are free to use this Item in any way that is permitted by the copyright and related rights legislation that applies to your use. For other uses you need to obtain permission from the rights-holder(s). https://rightsstatements.org/page/InC/1.0/;
    Full Citation
    Molecular weight characterization for glycosaminoglycans with different degrees of sulfation by size exclusion chromatography, Z. Wang, F. Zhang, J. S. Dordick, R. J. Linhardt, Current Analytical Chemistry, , 8, 506-511, 2012.
    Metadata
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    URI
    https://hdl.handle.net/20.500.13015/5306; https://doi.org/10.2174%2F157341112803216753
    Abstract
    Different degrees of glycosaminoglycan sulfation result in their different charge densities. The charge density differences impact their migration behavior in polyacrylamide gel electrophoresis and size exclusion chromatography, two of the most common methods for determining relative molecular masses of polysaccharides. In this study, we investigated the feasibility of using commercially available heparin oligosaccharides as calibrants for measuring the relative molecular masses of intermediates in a bioengineered heparin process that have different levels of sulfation. A size exclusion chromatography method was established that eliminates this charge density effect and allows the determination of relative molecular mass using a single calibration curve with heparin oligosaccharides calibrants. This is accomplished by overcoming the electrostatic interaction between the glycosaminoglycans and size exclusion chromatography stationary phase using high ionic strength mobile phase.;
    Description
    Current Analytical Chemistry, , 8, 506-511; Note : if this item contains full text it may be a preprint, author manuscript, or a Gold OA copy that permits redistribution with a license such as CC BY. The final version is available through the publisher’s platform.
    Department
    The Linhardt Research Labs.; The Shirley Ann Jackson, Ph.D. Center for Biotechnology and Interdisciplinary Studies (CBIS);
    Publisher
    Bentham Science
    Relationships
    The Linhardt Research Labs Online Collection; Rensselaer Polytechnic Institute, Troy, NY; https://harc.rpi.edu/;
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    A full text version is available in DSpace@RPI;
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