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dc.contributor.authorWang, Z.
dc.contributor.authorZhang, F.
dc.contributor.authorDordick, J.S.
dc.contributor.authorLinhardt, Robert J.
dc.date2012
dc.date.accessioned2022-06-23T04:14:44Z
dc.date.available2022-06-23T04:14:44Z
dc.date.issued2012
dc.identifier.citationMolecular weight characterization for glycosaminoglycans with different degrees of sulfation by size exclusion chromatography, Z. Wang, F. Zhang, J. S. Dordick, R. J. Linhardt, Current Analytical Chemistry, , 8, 506-511, 2012.
dc.identifier.urihttps://hdl.handle.net/20.500.13015/5306
dc.identifier.urihttps://doi.org/10.2174%2F157341112803216753
dc.descriptionCurrent Analytical Chemistry, , 8, 506-511
dc.descriptionNote : if this item contains full text it may be a preprint, author manuscript, or a Gold OA copy that permits redistribution with a license such as CC BY. The final version is available through the publisher’s platform.
dc.description.abstractDifferent degrees of glycosaminoglycan sulfation result in their different charge densities. The charge density differences impact their migration behavior in polyacrylamide gel electrophoresis and size exclusion chromatography, two of the most common methods for determining relative molecular masses of polysaccharides. In this study, we investigated the feasibility of using commercially available heparin oligosaccharides as calibrants for measuring the relative molecular masses of intermediates in a bioengineered heparin process that have different levels of sulfation. A size exclusion chromatography method was established that eliminates this charge density effect and allows the determination of relative molecular mass using a single calibration curve with heparin oligosaccharides calibrants. This is accomplished by overcoming the electrostatic interaction between the glycosaminoglycans and size exclusion chromatography stationary phase using high ionic strength mobile phase.
dc.languageen_US
dc.language.isoENG
dc.publisherBentham Science
dc.relation.ispartofThe Linhardt Research Labs Online Collection
dc.relation.ispartofRensselaer Polytechnic Institute, Troy, NY
dc.relation.urihttps://harc.rpi.edu/
dc.subjectBiology
dc.subjectChemistry and chemical biology
dc.subjectChemical and biological engineering
dc.subjectBiomedical engineering
dc.titleMolecular weight characterization for glycosaminoglycans with different degrees of sulfation by size exclusion chromatography
dc.typeArticle
dcterms.accessRightsA full text version is available in DSpace@RPI
dcterms.isVersionOfhttps://doi.org/10.2174%2F157341112803216753
dc.rights.holderIn Copyright : this Item is protected by copyright and/or related rights. You are free to use this Item in any way that is permitted by the copyright and related rights legislation that applies to your use. For other uses you need to obtain permission from the rights-holder(s). https://rightsstatements.org/page/InC/1.0/
dc.creator.identifierhttps://orcid.org/0000-0003-2219-5833
dc.relation.departmentThe Linhardt Research Labs.
dc.relation.departmentThe Shirley Ann Jackson, Ph.D. Center for Biotechnology and Interdisciplinary Studies (CBIS)


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