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    Complete mass spectral characterization of a synthetic ultralow molecular weight heparin using collision induced dissociation

    Author
    Kailemia, Muchena J.; Li, Lingyun; Ly, Mellisa; Linhardt, Robert J.; Amster, I. Jonathan
    ORCID
    https://orcid.org/0000-0003-2219-5833
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    COMPLETE MASS SPECTRAL CHARACTERIZATION OF A SYNTHETIC ULTRALOW.pdf (204.8Kb)
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    Date Issued
    2012-07-03
    Subject
    Biology; Chemistry and chemical biology; Chemical and biological engineering; Biomedical engineering
    Degree
    Terms of Use
    In Copyright : this Item is protected by copyright and/or related rights. You are free to use this Item in any way that is permitted by the copyright and related rights legislation that applies to your use. For other uses you need to obtain permission from the rights-holder(s). https://rightsstatements.org/page/InC/1.0/;
    Full Citation
    Complete mass spectral characterization of a synthetic ultralow molecular weight heparin using collision induced dissociation, M. J. Kailemia, L. Li, M. Ly, R. J. Linhardt, J. Amster, Analytical Chemistry, 84, 5475-5478, 2012.
    Metadata
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    URI
    https://hdl.handle.net/20.500.13015/5310; https://doi.org/10.1021/ac3015824
    Abstract
    Glycosaminoglycans (GAGs) are a class of biologically important molecules, and their structural analysis is the target of considerable research effort. Advances in tandem mass spectrometry (MS/MS) have recently enabled the structural characterization of several classes of GAGs; however, the highly sulfated GAGs, such as heparins, have remained a relatively intractable class due their tendency to lose SO3 during MS/MS, producing few sequence-informative fragment ions. The present work demonstrates for the first time the complete structural characterization of the highly sulfated heparin-based drug Arixtra. This was achieved by Na+/H+ exchange to create a more ionized species that was stable against SO3 loss, and that produced complete sets of both glycosidic and cross-ring fragment ions. MS/MS enables the complete structural determination of Arixtra, including the stereochemistry of its uronic acid residues, and suggests an approach for solving the structure of more complex, highly sulfated heparin-based drugs.;
    Description
    Analytical Chemistry, 84, 5475-5478; Note : if this item contains full text it may be a preprint, author manuscript, or a Gold OA copy that permits redistribution with a license such as CC BY. The final version is available through the publisher’s platform.
    Department
    The Linhardt Research Labs.; The Shirley Ann Jackson, Ph.D. Center for Biotechnology and Interdisciplinary Studies (CBIS);
    Publisher
    American Chemical Society (ACS)
    Relationships
    The Linhardt Research Labs Online Collection; Rensselaer Polytechnic Institute, Troy, NY; Analytical Chemistry; https://harc.rpi.edu/;
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    A full text version is available in DSpace@RPI;
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