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    Changes in glycosaminoglycan structure on differentiation of human embryonic stem cells towards mesoderm and endoderm lineages

    Author
    Gasimli, Leyla; Hickey, Anne Marie; Yang, Bo; Li, Guoyun; Dela Rosa, Mitche; Nairn, Alison V.; Kulik, Michael J.; Dordick, Jonathan S.; Moremen, Kelley W.; Dalton, Stephen; Linhardt, Robert J.
    ORCID
    https://orcid.org/0000-0003-2219-5833
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    CHANGES IN GLYCOSAMINOGLYCAN STRUCTURE ON DIFFERENTIATION OF.pdf (1.195Mb)
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    Date Issued
    2014-01-01
    Subject
    Biology; Chemistry and chemical biology; Chemical and biological engineering; Biomedical engineering
    Degree
    Terms of Use
    In Copyright : this Item is protected by copyright and/or related rights. You are free to use this Item in any way that is permitted by the copyright and related rights legislation that applies to your use. For other uses you need to obtain permission from the rights-holder(s). https://rightsstatements.org/page/InC/1.0/;
    Full Citation
    Changes in glycosaminoglycan structure on differentiation of human embryonic stem cells towards mesoderm and endoderm lineages, L. Gasimli, A. M. Hickey, B. Yang, G. Li, M. dela Rosa, A. V. Nairn, M. J. Kulik, J. S. Dordick, K. W. Moremen, S. Dalton, R. J. Linhardt, Biochemica et Biophysica Acta, 1840, 1993–2003, 2014.
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    URI
    https://hdl.handle.net/20.500.13015/5326; https://doi.org/10.1016/j.bbagen.2014.01.007
    Abstract
    Background: Proteoglycans are found on the cell surface and in the extracellular matrix, and serve as prime sites for interaction with signaling molecules. Proteoglycans help regulate pathways that control stem cell fate, and therefore represent an excellent tool to manipulate these pathways. Despite their importance, there is a dearth of data linking glycosaminoglycan structure within proteoglycans with stem cell differentiation. Methods: Human embryonic stem cell line WA09 (H9) was differentiated into early mesoderm and endoderm lineages, and the glycosaminoglycanomic changes accompanying these transitions were studied using transcript analysis, immunoblotting, immunofluorescence and disaccharide analysis. Results: Pluripotent H9 cell lumican had no glycosaminoglycan chains whereas in splanchnic mesoderm lumican was glycosaminoglycanated. H9 cells have primarily non-sulfated heparan sulfate chains. On differentiation towards splanchnic mesoderm and hepatic lineages N-sulfo group content increases. Differences in transcript expression of NDST1, HS6ST2 and HS6ST3, three heparan sulfate biosynthetic enzymes, within splanchnic mesoderm cells compared to H9 cells correlate to changes in glycosaminoglycan structure. Conclusions: Differentiation of embryonic stem cells markedly changes the proteoglycanome. General significance: The glycosaminoglycan biosynthetic pathway is complex and highly regulated, and therefore, understanding the details of this pathway should enable better control with the aim of directing stem cell differentiation.;
    Description
    Biochemica et Biophysica Acta, 1840, 1993–2003; Note : if this item contains full text it may be a preprint, author manuscript, or a Gold OA copy that permits redistribution with a license such as CC BY. The final version is available through the publisher’s platform.
    Department
    The Linhardt Research Labs.; The Shirley Ann Jackson, Ph.D. Center for Biotechnology and Interdisciplinary Studies (CBIS);
    Publisher
    Elsevier
    Relationships
    The Linhardt Research Labs Online Collection; Rensselaer Polytechnic Institute, Troy, NY; Biochimica et Biophysica Acta - General Subjects; https://harc.rpi.edu/;
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    A full text version is available in DSpace@RPI;
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