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dc.contributor.authorGasimli, Leyla
dc.contributor.authorHickey, Anne Marie
dc.contributor.authorYang, Bo
dc.contributor.authorLi, Guoyun
dc.contributor.authorDela Rosa, Mitche
dc.contributor.authorNairn, Alison V.
dc.contributor.authorKulik, Michael J.
dc.contributor.authorDordick, Jonathan S.
dc.contributor.authorMoremen, Kelley W.
dc.contributor.authorDalton, Stephen
dc.contributor.authorLinhardt, Robert J.
dc.date2014
dc.date.accessioned2022-06-23T04:17:32Z
dc.date.available2022-06-23T04:17:32Z
dc.date.issued2014-01-01
dc.identifier.citationChanges in glycosaminoglycan structure on differentiation of human embryonic stem cells towards mesoderm and endoderm lineages, L. Gasimli, A. M. Hickey, B. Yang, G. Li, M. dela Rosa, A. V. Nairn, M. J. Kulik, J. S. Dordick, K. W. Moremen, S. Dalton, R. J. Linhardt, Biochemica et Biophysica Acta, 1840, 1993–2003, 2014.
dc.identifier.issn18728006
dc.identifier.issn3044165
dc.identifier.urihttps://hdl.handle.net/20.500.13015/5326
dc.identifier.urihttps://doi.org/10.1016/j.bbagen.2014.01.007
dc.descriptionBiochemica et Biophysica Acta, 1840, 1993–2003
dc.descriptionNote : if this item contains full text it may be a preprint, author manuscript, or a Gold OA copy that permits redistribution with a license such as CC BY. The final version is available through the publisher’s platform.
dc.description.abstractBackground: Proteoglycans are found on the cell surface and in the extracellular matrix, and serve as prime sites for interaction with signaling molecules. Proteoglycans help regulate pathways that control stem cell fate, and therefore represent an excellent tool to manipulate these pathways. Despite their importance, there is a dearth of data linking glycosaminoglycan structure within proteoglycans with stem cell differentiation. Methods: Human embryonic stem cell line WA09 (H9) was differentiated into early mesoderm and endoderm lineages, and the glycosaminoglycanomic changes accompanying these transitions were studied using transcript analysis, immunoblotting, immunofluorescence and disaccharide analysis. Results: Pluripotent H9 cell lumican had no glycosaminoglycan chains whereas in splanchnic mesoderm lumican was glycosaminoglycanated. H9 cells have primarily non-sulfated heparan sulfate chains. On differentiation towards splanchnic mesoderm and hepatic lineages N-sulfo group content increases. Differences in transcript expression of NDST1, HS6ST2 and HS6ST3, three heparan sulfate biosynthetic enzymes, within splanchnic mesoderm cells compared to H9 cells correlate to changes in glycosaminoglycan structure. Conclusions: Differentiation of embryonic stem cells markedly changes the proteoglycanome. General significance: The glycosaminoglycan biosynthetic pathway is complex and highly regulated, and therefore, understanding the details of this pathway should enable better control with the aim of directing stem cell differentiation.
dc.description.sponsorshipNational Institutes of Health
dc.languageen_US
dc.language.isoENG
dc.publisherElsevier
dc.relation.ispartofThe Linhardt Research Labs Online Collection
dc.relation.ispartofRensselaer Polytechnic Institute, Troy, NY
dc.relation.ispartofBiochimica et Biophysica Acta - General Subjects
dc.relation.urihttps://harc.rpi.edu/
dc.subjectBiology
dc.subjectChemistry and chemical biology
dc.subjectChemical and biological engineering
dc.subjectBiomedical engineering
dc.titleChanges in glycosaminoglycan structure on differentiation of human embryonic stem cells towards mesoderm and endoderm lineages
dc.typeArticle
dcterms.accessRightsA full text version is available in DSpace@RPI
dcterms.isPartOfJournal
dcterms.isVersionOfhttps://doi.org/10.1016/j.bbagen.2014.01.007
dc.rights.holderIn Copyright : this Item is protected by copyright and/or related rights. You are free to use this Item in any way that is permitted by the copyright and related rights legislation that applies to your use. For other uses you need to obtain permission from the rights-holder(s). https://rightsstatements.org/page/InC/1.0/
dc.creator.identifierhttps://orcid.org/0000-0003-2219-5833
dc.relation.departmentThe Linhardt Research Labs.
dc.relation.departmentThe Shirley Ann Jackson, Ph.D. Center for Biotechnology and Interdisciplinary Studies (CBIS)
rpi.description.pages1993-2003
rpi.description.volume1840


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