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    Analysis of heparin oligosaccharides by capillary electrophoresis- negative-ion electrospray ionization mass spectrometry

    Author
    Lin, Lei; Liu, Xinyue; Zhang, Fuming; Chi, Lianli; Amster, I. Jonathan; Leach, Franklyn E.; Xia, Qiangwei; Linhardt, Robert J.
    ORCID
    https://orcid.org/0000-0003-2219-5833
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    ANALYSIS OF HEPARIN OLIGOSACCHARIDES BY CAPILLARY.pdf (2.306Mb)
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    Date Issued
    2017-01-01
    Subject
    Biology; Chemistry and chemical biology; Chemical and biological engineering; Biomedical engineering
    Degree
    Terms of Use
    In Copyright : this Item is protected by copyright and/or related rights. You are free to use this Item in any way that is permitted by the copyright and related rights legislation that applies to your use. For other uses you need to obtain permission from the rights-holder(s). https://rightsstatements.org/page/InC/1.0/;
    Full Citation
    Analysis of heparin oligosaccharides by capillary electrophoresis- negative-ion electrospray ionization mass spectrometry, L. Lin, X. Liu, F. Zhang, L. Chi, I. J. Amster, F. E. Leach III, Q. Xia, R. J. Linhardt, Analytical and Bioanalytical Chemistry,409, 411–420, 2017.
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    URI
    https://hdl.handle.net/20.500.13015/5341; https://doi.org/10.1007/s00216-016-9662-1
    Abstract
    Most hyphenated analytical approaches that rely on liquid chromatography–MS require relatively long separation times, produce incomplete resolution of oligosaccharide mixtures, use eluents that are incompatible with electrospray ionization, or require oligosaccharide derivatization. Here we demonstrate the analysis of heparin oligosaccharides, including disaccharides, ultralow molecular weight heparin, and a low molecular weight heparin, using a novel electrokinetic pump-based CE–MS coupling eletrospray ion source. Reverse polarity CE separation and negative-mode electrospray ionization were optimized using a volatile methanolic ammonium acetate electrolyte and sheath fluid. The online CE hyphenated negative-ion electrospray ionization MS on an LTQ Orbitrap mass spectrometer was useful in disaccharide compositional analysis and bottom-up and top-down analysis of low molecular weight heparin. The application of this CE–MS method to ultralow molecular heparin suggests that a charge state distribution and the low level of sulfate group loss that is achieved make this method useful for online tandem MS analysis of heparins.;
    Description
    Analytical and Bioanalytical Chemistry,409, 411–420; Note : if this item contains full text it may be a preprint, author manuscript, or a Gold OA copy that permits redistribution with a license such as CC BY. The final version is available through the publisher’s platform.
    Department
    The Linhardt Research Labs.; The Shirley Ann Jackson, Ph.D. Center for Biotechnology and Interdisciplinary Studies (CBIS);
    Publisher
    Springer
    Relationships
    The Linhardt Research Labs Online Collection; Rensselaer Polytechnic Institute, Troy, NY; Analytical and Bioanalytical Chemistry; https://harc.rpi.edu/;
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    A full text version is available in DSpace@RPI;
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