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    A purification process for heparin and precursor polysaccharides using the pH responsive behavior of chitosan

    Author
    Bhaskar, Ujjwal; Hickey, Anne M.; Li, Guoyun; Mundra, Ruchir V.; Zhang, Fuming; Fu, Li; Cai, Chao; Ou, Zhimin; Dordick, Jonathan S.; Linhardt, Robert J.
    ORCID
    https://orcid.org/0000-0003-2219-5833
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    A PURIFICATION PROCESS FOR HEPARIN AND PRECURSOR.pdf (963.6Kb)
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    Date Issued
    2015-09-01
    Subject
    Biology; Chemistry and chemical biology; Chemical and biological engineering; Biomedical engineering
    Degree
    Terms of Use
    In Copyright : this Item is protected by copyright and/or related rights. You are free to use this Item in any way that is permitted by the copyright and related rights legislation that applies to your use. For other uses you need to obtain permission from the rights-holder(s). https://rightsstatements.org/page/InC/1.0/;
    Full Citation
    A purification process for heparin and precursor polysaccharides using the pH responsive behavior of chitosan, U. Bhaskar, A.M. Hickey, G. Li, C. Cai, L. Fu, Z. Ou, R. V. Mundra, F. Zhang, J. S. Dordick, R. J. Linhardt, Biotechnology Progress, 31, 1348–1359, 2015.
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    URI
    https://hdl.handle.net/20.500.13015/5352; https://doi.org/10.1002/btpr.2144
    Abstract
    The contamination crisis of 2008 has brought to light several risks associated with use of animal tissue derived heparin. Because the total chemical synthesis of heparin is not feasible, a bioengineered approach has been proposed, relying on recombinant enzymes derived from the heparin/HS biosynthetic pathway and Escherichia coli K5 capsular polysaccharide. Intensive process engineering efforts are required to achieve a cost-competitive process for bioengineered heparin compared to commercially available porcine heparins. Towards this goal, we have used 96-well plate based screening for development of a chitosan-based purification process for heparin and precursor polysaccharides. The unique pH responsive behavior of chitosan enables simplified capture of target heparin or related polysaccharides, under low pH and complex solution conditions, followed by elution under mildly basic conditions. The use of mild, basic recovery conditions are compatible with the chemical Ndeacetylation/N-sulfonation step used in the bioengineered heparin process. Selective precipitation of glycosaminoglycans (GAGs) leads to significant removal of process related impurities such as proteins, DNA and endotoxins. Use of highly sensitive liquid chromatographymass spectrometry and nuclear magnetic resonance analytical techniques reveal a minimum impact of chitosan-based purification on heparin product composition.;
    Description
    Biotechnology Progress, 31, 1348–1359; Note : if this item contains full text it may be a preprint, author manuscript, or a Gold OA copy that permits redistribution with a license such as CC BY. The final version is available through the publisher’s platform.
    Department
    The Linhardt Research Labs.; The Shirley Ann Jackson, Ph.D. Center for Biotechnology and Interdisciplinary Studies (CBIS);
    Publisher
    Wiley
    Relationships
    The Linhardt Research Labs Online Collection; Rensselaer Polytechnic Institute, Troy, NY; Biotechnology Progress; https://harc.rpi.edu/;
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    A full text version is available in DSpace@RPI;
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