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dc.contributor.authorBhaskar, Ujjwal
dc.contributor.authorHickey, Anne M.
dc.contributor.authorLi, Guoyun
dc.contributor.authorMundra, Ruchir V.
dc.contributor.authorZhang, Fuming
dc.contributor.authorFu, Li
dc.contributor.authorCai, Chao
dc.contributor.authorOu, Zhimin
dc.contributor.authorDordick, Jonathan S.
dc.contributor.authorLinhardt, Robert J.
dc.date2015
dc.date.accessioned2022-06-23T04:20:08Z
dc.date.available2022-06-23T04:20:08Z
dc.date.issued2015-09-01
dc.identifier.citationA purification process for heparin and precursor polysaccharides using the pH responsive behavior of chitosan, U. Bhaskar, A.M. Hickey, G. Li, C. Cai, L. Fu, Z. Ou, R. V. Mundra, F. Zhang, J. S. Dordick, R. J. Linhardt, Biotechnology Progress, 31, 1348–1359, 2015.
dc.identifier.issn15206033
dc.identifier.issn87567938
dc.identifier.urihttps://hdl.handle.net/20.500.13015/5352
dc.identifier.urihttps://doi.org/10.1002/btpr.2144
dc.descriptionBiotechnology Progress, 31, 1348–1359
dc.descriptionNote : if this item contains full text it may be a preprint, author manuscript, or a Gold OA copy that permits redistribution with a license such as CC BY. The final version is available through the publisher’s platform.
dc.description.abstractThe contamination crisis of 2008 has brought to light several risks associated with use of animal tissue derived heparin. Because the total chemical synthesis of heparin is not feasible, a bioengineered approach has been proposed, relying on recombinant enzymes derived from the heparin/HS biosynthetic pathway and Escherichia coli K5 capsular polysaccharide. Intensive process engineering efforts are required to achieve a cost-competitive process for bioengineered heparin compared to commercially available porcine heparins. Towards this goal, we have used 96-well plate based screening for development of a chitosan-based purification process for heparin and precursor polysaccharides. The unique pH responsive behavior of chitosan enables simplified capture of target heparin or related polysaccharides, under low pH and complex solution conditions, followed by elution under mildly basic conditions. The use of mild, basic recovery conditions are compatible with the chemical Ndeacetylation/N-sulfonation step used in the bioengineered heparin process. Selective precipitation of glycosaminoglycans (GAGs) leads to significant removal of process related impurities such as proteins, DNA and endotoxins. Use of highly sensitive liquid chromatographymass spectrometry and nuclear magnetic resonance analytical techniques reveal a minimum impact of chitosan-based purification on heparin product composition.
dc.description.sponsorshipNational Heart, Lung, and Blood Institute
dc.languageen_US
dc.language.isoENG
dc.publisherWiley
dc.relation.ispartofThe Linhardt Research Labs Online Collection
dc.relation.ispartofRensselaer Polytechnic Institute, Troy, NY
dc.relation.ispartofBiotechnology Progress
dc.relation.urihttps://harc.rpi.edu/
dc.subjectBiology
dc.subjectChemistry and chemical biology
dc.subjectChemical and biological engineering
dc.subjectBiomedical engineering
dc.titleA purification process for heparin and precursor polysaccharides using the pH responsive behavior of chitosan
dc.typeArticle
dcterms.accessRightsA full text version is available in DSpace@RPI
dcterms.isPartOfJournal
dcterms.isVersionOfhttps://doi.org/10.1002/btpr.2144
dc.rights.holderIn Copyright : this Item is protected by copyright and/or related rights. You are free to use this Item in any way that is permitted by the copyright and related rights legislation that applies to your use. For other uses you need to obtain permission from the rights-holder(s). https://rightsstatements.org/page/InC/1.0/
dc.creator.identifierhttps://orcid.org/0000-0003-2219-5833
dc.relation.departmentThe Linhardt Research Labs.
dc.relation.departmentThe Shirley Ann Jackson, Ph.D. Center for Biotechnology and Interdisciplinary Studies (CBIS)
rpi.description.pages1348-1359
rpi.description.volume31


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