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dc.contributor.authorLi, Guoyun
dc.contributor.authorLi, Lingyun
dc.contributor.authorXue, Changhu
dc.contributor.authorMiddleton, Dustin
dc.contributor.authorLinhardt, Robert J.
dc.contributor.authorAvci, Fikri Y.
dc.date2015
dc.date.accessioned2022-06-23T04:20:09Z
dc.date.available2022-06-23T04:20:09Z
dc.date.issued2015-06-05
dc.identifier.citationProfiling pneumococcal type 3-derived oligosaccharides by high resolution liquid chromatography-tandem mass spectrometry, G. Li, L. Li, C. Xue, D. Middleton, R. J. Linhardt, F. Y. Avci, Journal of Chromatography A, 1397, 43–51, 2015.
dc.identifier.issn18733778
dc.identifier.issn219673
dc.identifier.urihttps://hdl.handle.net/20.500.13015/5356
dc.identifier.urihttps://doi.org/10.1016/j.chroma.2015.04.009
dc.descriptionJournal of Chromatography A, 1397, 43–51
dc.descriptionNote : if this item contains full text it may be a preprint, author manuscript, or a Gold OA copy that permits redistribution with a license such as CC BY. The final version is available through the publisher’s platform.
dc.description.abstractPneumococcal type-3 polysaccharide (Pn3P) is considered a major target for the development of a human vaccine to protect against Streptococcus pneumoniae infection. Thus, it is critical to develop methods for the preparation and analysis of Pn3P-derived oligosaccharides to better understand its immunological properties. In this paper, we profile oligosaccharides, generated by the free radical depolymerization of Pn3P, using liquid chromatography (LC)-tandem mass spectrometry (MS/MS). Hydrophilic liquid interaction chromatography (HILIC)-mass spectrometry (MS) revealed a series of oligosaccharides with an even- and odd-number of saccharide residues, ranging from monosaccharide, degree of polymerization (dp1) to large oligosaccharides up to dp 20, generated by free radical depolymerization. Isomers of oligosaccharides with an even number of sugar residues were easily separated on a HILIC column, and their sequences could be distinguished by comparing MS/MS of these oligosaccharides and their reduced alditols. Fluorescent labeling with 2-aminoacridone (AMAC) followed by reversed phase (RP)-LC-MS/MS was applied to analyze and sequence poorly separated product mixtures, as RP-LC affords higher resolution of AMAC-labeled oligosaccharides than does HILIC-based separation. The present methodology can be potentially applied to profiling other capsular polysaccharides.
dc.description.sponsorshipNational Institutes of Health
dc.languageen_US
dc.language.isoENG
dc.publisherElsevier
dc.relation.ispartofThe Linhardt Research Labs Online Collection
dc.relation.ispartofRensselaer Polytechnic Institute, Troy, NY
dc.relation.ispartofJournal of Chromatography A
dc.relation.urihttps://harc.rpi.edu/
dc.subjectBiology
dc.subjectChemistry and chemical biology
dc.subjectChemical and biological engineering
dc.subjectBiomedical engineering
dc.titleProfiling pneumococcal type 3-derived oligosaccharides by high resolution liquid chromatography-tandem mass spectrometry
dc.typeArticle
dcterms.accessRightsA full text version is available in DSpace@RPI
dcterms.isPartOfJournal
dcterms.isVersionOfhttps://doi.org/10.1016/j.chroma.2015.04.009
dc.rights.holderIn Copyright : this Item is protected by copyright and/or related rights. You are free to use this Item in any way that is permitted by the copyright and related rights legislation that applies to your use. For other uses you need to obtain permission from the rights-holder(s). https://rightsstatements.org/page/InC/1.0/
dc.creator.identifierhttps://orcid.org/0000-0003-2219-5833
dc.relation.departmentThe Linhardt Research Labs.
dc.relation.departmentThe Shirley Ann Jackson, Ph.D. Center for Biotechnology and Interdisciplinary Studies (CBIS)
rpi.description.pages43-51
rpi.description.volume1397


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