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    Stable-isotopic analysis of porcine, bovine, and ovine heparins

    Author
    Jasper, John P.; Zhang, Fuming; Poe, Russell B.; Linhardt, Robert J.
    ORCID
    https://orcid.org/0000-0003-2219-5833
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    STABLE-ISOTOPIC ANALYSIS OF PORCINE, BOVINE, AND OVINE HEPARINS.pdf (2.198Mb)
    Other Contributors
    Date Issued
    2015-01-01
    Subject
    Biology; Chemistry and chemical biology; Chemical and biological engineering; Biomedical engineering
    Degree
    Terms of Use
    In Copyright : this Item is protected by copyright and/or related rights. You are free to use this Item in any way that is permitted by the copyright and related rights legislation that applies to your use. For other uses you need to obtain permission from the rights-holder(s). https://rightsstatements.org/page/InC/1.0/;
    Full Citation
    Stable-isotopic analysis of porcine, bovine, and ovine heparins, J. P. Jasper, F. Zhang, R. B. Poe, R. J. Linhardt, Journal of Pharmaceutical Sciences,104, 457–463, 2015
    Metadata
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    URI
    https://hdl.handle.net/20.500.13015/5360; https://doi.org/10.1002/jps.24134
    Abstract
    The assessment of provenance of heparin is becoming a major concern for the pharmaceutical industry and its regulatory bodies. Batch-specific [carbon (δ13C), nitrogen (δ15N), oxygen (δ18O), sulfur (δ34S), and hydrogen (δD)] stable-isotopic compositions of five different animal-derived heparins were performed. Measurements readily allowed their differentiation into groups and/or subgroups based on their isotopic provenance. Principle component analysis showed that a bivariate plot of δ13C and δ18O is the best single, bivariate plot that results in the maximum discrimination ability when only two stable isotopes are used to describe the variation in the data set. Stable-isotopic analyses revealed that (i) stable-isotope measurements on these highly-sulfated polysaccharide (MW ~15 kDa) natural products (“biologics”) were feasible; (ii) in bivariate plots, the δ13C versus δ18O plot reveals a well-defined relationship for source differentiation of hogs raised in the US from hogs raised in Europe and China; (iii) the δD versus δ18O plot revealed the most well-defined relationship for source differentiation based on the hydrologic-environmental isotopes of water (D/H and 18O/16O), and (iv) the δ15N versus δ18O and δ34S versus δ18O relationships are both very similar, possibly reflecting the food sources used by the different heparin producers.;
    Description
    Journal of Pharmaceutical Sciences,104, 457–463; Note : if this item contains full text it may be a preprint, author manuscript, or a Gold OA copy that permits redistribution with a license such as CC BY. The final version is available through the publisher’s platform.
    Department
    The Linhardt Research Labs.; The Shirley Ann Jackson, Ph.D. Center for Biotechnology and Interdisciplinary Studies (CBIS);
    Publisher
    Elsevier
    Relationships
    The Linhardt Research Labs Online Collection; Rensselaer Polytechnic Institute, Troy, NY; Journal of Pharmaceutical Sciences; https://harc.rpi.edu/;
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    A full text version is available in DSpace@RPI;
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