Click-coated, heparinized, decellularized vascular grafts
Authors
Dimitrievska, Sashka
Cai, Chao
Weyers, Amanda
Balestrini, Jenna L.
Lin, Tylee
Sundaram, Sumati
Hatachi, Go
Spiegel, David A.
Kyriakides, Themis R.
Miao, Jianjun
ORCID
https://orcid.org/0000-0003-2219-5833
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Other Contributors
Issue Date
2015-02-01
Keywords
Biology , Chemistry and chemical biology , Chemical and biological engineering , Biomedical engineering
Degree
Terms of Use
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Full Citation
Click-coated, heparinized, decellularized vascular grafts, S. Dimitrievska, C. Cai, A. Weyers, T. Lin, S. Sundaram, D. A. Spiegel, T. R. Kyriakides, J. Miao, G. Li, L. Niklason, R. J. Linhardt, Acta Biomateriala, 13, 177–187, 2015.
Abstract
A novel method enabling the engineering of a dense and appropriately oriented heparin-containing layer on decellularized aortas has been developed. Amino groups of decellularized aortas were first modified to azido groups using 3-azidobenzoic acid. Azide-clickable dendrons were attached onto the azido groups through "alkyne-azide" click chemistry, affording a tenfold amplification of adhesions sites. Dendron end groups were finally decorated with end-on modified heparin chains. Heparin chains were oriented like heparan sulfate groups on native endothelial cells surface. X-ray photoelectron spectroscopy, nuclear magnetic resonance imaging, mass spectrometry and Fourier transform infrared FTIR spectroscopy were used to characterize the synthesis steps, building the final heparin layered coatings. The continuity of the heparin coating was verified using fluorescent microscopy and histological analysis. The efficacy of heparin linkage was demonstrated with factor Xa anti-thrombogenic assay and platelet adhesion studies. The results suggest that oriented heparin immobilization to decellularized aortas may improve the in vivo blood compatibility of decellularized aortas and vessels.
Description
Acta Biomateriala, 13, 177–187
Note : if this item contains full text it may be a preprint, author manuscript, or a Gold OA copy that permits redistribution with a license such as CC BY. The final version is available through the publisher’s platform.
Note : if this item contains full text it may be a preprint, author manuscript, or a Gold OA copy that permits redistribution with a license such as CC BY. The final version is available through the publisher’s platform.
Department
The Linhardt Research Labs.
The Shirley Ann Jackson, Ph.D. Center for Biotechnology and Interdisciplinary Studies (CBIS)
The Shirley Ann Jackson, Ph.D. Center for Biotechnology and Interdisciplinary Studies (CBIS)
Publisher
Elsevier
Relationships
The Linhardt Research Labs Online Collection
Rensselaer Polytechnic Institute, Troy, NY
Acta Biomaterialia
https://harc.rpi.edu/
Rensselaer Polytechnic Institute, Troy, NY
Acta Biomaterialia
https://harc.rpi.edu/
Access
A full text version is available in DSpace@RPI