dc.rights.license | Open Access | |
dc.contributor.author | Cress, Brady F. | |
dc.contributor.author | Bhaskar, Ujjwal | |
dc.contributor.author | Vaidyanathan, Deepika | |
dc.contributor.author | Williams, Asher | |
dc.contributor.author | Cai, Chao | |
dc.contributor.author | Liu, Xinyue | |
dc.contributor.author | Fu, Li | |
dc.contributor.author | M-Chari, Vandhana | |
dc.contributor.author | Zhang, Fuming | |
dc.contributor.author | Mousa, Shaker A. | |
dc.contributor.author | Dordick, Jonathan S. | |
dc.contributor.author | Koffas, Mattheos A.G. | |
dc.contributor.author | Linhardt, Robert J. | |
dc.date | 2019 | |
dc.date.accessioned | 2022-06-23T04:28:40Z | |
dc.date.available | 2022-06-23T04:28:40Z | |
dc.date.issued | 2019-04-23 | |
dc.identifier.citation | Heavy heparin: A stable isotope-enriched, chemoenzymatically-synthesized, poly-component drug, B. F. Cress, U. Bhaskar, D. Vaidyanathan, A. Williams, C. Cai, X. Liu, L. Fu, V. M-Chari, F. Zhang, S. A. Mousa, J. S. Dordick, M. A. G. Koffas, R. J. Linhardt, Angewandte Chemie, 58, 5962 –5966, 2019. | |
dc.identifier.issn | 15213773 | |
dc.identifier.issn | 14337851 | |
dc.identifier.uri | https://hdl.handle.net/20.500.13015/5367 | |
dc.identifier.uri | https://doi.org/10.1002/anie.201900768 | |
dc.description | Angewandte Chemie, 58, 5962 –5966 | |
dc.description | Note : if this item contains full text it may be a preprint, author manuscript, or a Gold OA copy that permits redistribution with a license such as CC BY. The final version is available through the publisher’s platform. | |
dc.description.abstract | Heparin is a highly sulfated, complex polysaccharide and widely used anticoagulant pharmaceutical. In this work, we chemoenzymatically synthesized perdeuteroheparin from biosynthetically enriched heparosan precursor obtained from microbial culture in deuterated medium. Chemical de-N-acetylation, chemical N-sulfation, enzymatic epimerization, and enzymatic sulfation with recombinant heparin biosynthetic enzymes afforded perdeuteroheparin comparable to pharmaceutical heparin. A series of applications for heavy heparin and its heavy biosynthetic intermediates are demonstrated, including generation of stable isotope labeled disaccharide standards, development of a non-radioactive NMR assay for glucuronosyl-C5-epimerase, and background-free quantification of in vivo half-life following administration to rabbits. We anticipate that this approach can be extended to produce other isotope-enriched glycosaminoglycans. | |
dc.description.sponsorship | National Science Foundation | |
dc.language | en_US | |
dc.language.iso | ENG | |
dc.publisher | Wiley | |
dc.relation.ispartof | The Linhardt Research Labs Online Collection | |
dc.relation.ispartof | Rensselaer Polytechnic Institute, Troy, NY | |
dc.relation.ispartof | Angewandte Chemie - International Edition | |
dc.relation.uri | https://harc.rpi.edu/ | |
dc.subject | Biology | |
dc.subject | Chemistry and chemical biology | |
dc.subject | Chemical and biological engineering | |
dc.subject | Biomedical engineering | |
dc.title | Heavy heparin: A stable isotope-enriched, chemoenzymatically-synthesized, poly-component drug | |
dc.type | Article | |
dcterms.accessRights | A full text version is available in DSpace@RPI | |
dcterms.isPartOf | Journal | |
dcterms.isVersionOf | https://doi.org/10.1002/anie.201900768 | |
dc.rights.holder | In Copyright : this Item is protected by copyright and/or related rights. You are free to use this Item in any way that is permitted by the copyright and related rights legislation that applies to your use. For other uses you need to obtain permission from the rights-holder(s). https://rightsstatements.org/page/InC/1.0/ | |
dc.creator.identifier | https://orcid.org/0000-0003-2219-5833 | |
dc.relation.department | The Linhardt Research Labs. | |
dc.relation.department | The Shirley Ann Jackson, Ph.D. Center for Biotechnology and Interdisciplinary Studies (CBIS) | |
rpi.description.pages | 5962-5966 | |
rpi.description.volume | 58 | |