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dc.rights.licenseOpen Access
dc.contributor.authorZhi, Zijian
dc.contributor.authorLi, Junhui
dc.contributor.authorChen, Jianle
dc.contributor.authorLi, Shan
dc.contributor.authorCheng, Huan
dc.contributor.authorLiu, Donghong
dc.contributor.authorYe, Xingqian
dc.contributor.authorLinhardt, Robert J.
dc.contributor.authorChen, Shiguo
dc.date2019
dc.date.accessioned2022-06-23T04:28:41Z
dc.date.available2022-06-23T04:28:41Z
dc.date.issued2019-04-01
dc.identifier.citationPreparation of low molecular weight heparin using an ultrasound-assisted Fenton-system, Z. Zhi, J. Li, J. Chen, S. Li, H. Cheng, D. Liu, X. Ye, R. J. Linhardt, S. Chen, Ultrasonics Sonochemistry, 52, 184–192, 2019.
dc.identifier.issn18732828
dc.identifier.issn13504177
dc.identifier.urihttps://hdl.handle.net/20.500.13015/5368
dc.identifier.urihttps://doi.org/10.1016/j.ultsonch.2018.11.016
dc.descriptionUltrasonics Sonochemistry, 52, 184–192
dc.descriptionNote : if this item contains full text it may be a preprint, author manuscript, or a Gold OA copy that permits redistribution with a license such as CC BY. The final version is available through the publisher’s platform.
dc.description.abstractHeparin, a high-molecular weight acidic polysaccharide, has raised much interest in the field of biomedical research due to its multiple bio-functions. The anticoagulant application of heparin in routine clinical practice, however, has been limited as the large molecular size of heparin can reduce its subcutaneous bioavailability and lead to severe adverse consequences such as thrombocytopenia. Here, we report a highly efficient and convenient method to depolymerize high-molecular weight, unfractionated heparin (UFH), into low molecular weight heparin (LMWH) by combining physical ultrasonic treatment with the chemical Fenton reaction, referred to as sono-Fenton. We found that this combination treatment synergistically degraded UFH into a LMWH of 4.87 kDa within 20 min. We characterized the mechanism of sono-Fenton heparin degradation through multiple approaches, including HPLC-SAX, disaccharide composition, FT-IR, NMR and top-down analysis, and found that the uronic acid residue in heparin was the most susceptible site attacked by OH radicals produced in the sono-Fenton process. Importantly, the LMWH prepared by this method had significantly higher anticoagulant activity than UFH and other LMWHs. This approach represents an effective method to produce heparin with improved activity and should be potentially useful for heparin production in the pharmaceutical industry.
dc.description.sponsorshipNational Institutes of Health
dc.languageen_US
dc.language.isoENG
dc.publisherElsevier
dc.relation.ispartofThe Linhardt Research Labs Online Collection
dc.relation.ispartofRensselaer Polytechnic Institute, Troy, NY
dc.relation.ispartofUltrasonics Sonochemistry
dc.relation.urihttps://harc.rpi.edu/
dc.subjectBiology
dc.subjectChemistry and chemical biology
dc.subjectChemical and biological engineering
dc.subjectBiomedical engineering
dc.titlePreparation of low molecular weight heparin using an ultrasound-assisted Fenton-system
dc.typeArticle
dcterms.accessRightsA full text version is available in DSpace@RPI
dcterms.isPartOfJournal
dcterms.isVersionOfhttps://doi.org/10.1016/j.ultsonch.2018.11.016
dc.rights.holderIn Copyright : this Item is protected by copyright and/or related rights. You are free to use this Item in any way that is permitted by the copyright and related rights legislation that applies to your use. For other uses you need to obtain permission from the rights-holder(s). https://rightsstatements.org/page/InC/1.0/
dc.creator.identifierhttps://orcid.org/0000-0003-2219-5833
dc.relation.departmentThe Linhardt Research Labs.
dc.relation.departmentThe Shirley Ann Jackson, Ph.D. Center for Biotechnology and Interdisciplinary Studies (CBIS)
rpi.description.pages184-192
rpi.description.volume52


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