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dc.rights.licenseACS AuthorChoice License
dc.contributor.authorGupta, Mamta
dc.contributor.authorZha, Jian
dc.contributor.authorZhang, Xing
dc.contributor.authorJung, Gyoo Yeol
dc.contributor.authorLinhardt, Robert J.
dc.contributor.authorKoffas, Mattheos A.G.
dc.date2018
dc.date.accessioned2022-06-23T04:28:42Z
dc.date.available2022-06-23T04:28:42Z
dc.date.issued2018-09-30
dc.identifier.citationProduction of deuterated cyanidin 3-O-glucoside from recombinant Escherichia coli M. Gupta, J. Zha, X. Zhang, G.-Y. Jung, R. J. Linhardt, M. Koffas, ACS Omega, 3, 11643−11648, 2018.
dc.identifier.issn24701343
dc.identifier.urihttps://hdl.handle.net/20.500.13015/5373
dc.identifier.urihttps://doi.org/10.1021/acsomega.8b01134
dc.descriptionACS Omega, 3, 11643−11648
dc.descriptionNote : if this item contains full text it may be a preprint, author manuscript, or a Gold OA copy that permits redistribution with a license such as CC BY. The final version is available through the publisher’s platform.
dc.description.abstractAnthocyanins are plant secondary metabolites that, despite their chemical instability, have found many applications as natural food colorants. They are also known for their beneficial health effects because of their antioxidant and anticancer properties. More stable versions of these molecules, particularly at neutral pH conditions, are required to study the anthocyanin pharmacokinetic properties and obtain effective therapeutic results. In the present report, a cost-effective technique was developed to prepare the deuterated anthocyanin using recombinant Escherichia coli as a production host and deuterated glycerol and D2O in the culture media. This approach resulted in the formation of endogenous deuterated uridine 5′-diphosphate-glucose that was further incorporated by the recombinant anthocyanin pathway, resulting in the formation of deuterated cyanidin 3-O-glucoside (C3G). The deuterium exchange of O–D and C–D were studied by liquid chromatography (LC)–mass spectrometry and NMR analysis. The labeled C3G, purified by high-performance LC showed a stable nature at pH 7.0 as compared to nondeuterated C3G.
dc.description.sponsorshipNational Science Foundation
dc.languageen_US
dc.language.isoENG
dc.publisherAmerican Chemical Society (ACS)
dc.relation.ispartofThe Linhardt Research Labs Online Collection
dc.relation.ispartofRensselaer Polytechnic Institute, Troy, NY
dc.relation.ispartofACS Omega
dc.relation.urihttps://harc.rpi.edu/
dc.subjectBiology
dc.subjectChemistry and chemical biology
dc.subjectChemical and biological engineering
dc.subjectBiomedical engineering
dc.titleProduction of deuterated cyanidin 3-O-glucoside from recombinant Escherichia coli
dc.typeArticle
dcterms.accessRightsA full text version is available in DSpace@RPI
dcterms.isPartOfJournal
dcterms.isVersionOfhttps://doi.org/10.1021/acsomega.8b01134
dc.rights.holderIn Copyright : this Item is protected by copyright and/or related rights. You are free to use this Item in any way that is permitted by the copyright and related rights legislation that applies to your use. For other uses you need to obtain permission from the rights-holder(s). https://rightsstatements.org/page/InC/1.0/
dc.creator.identifierhttps://orcid.org/0000-0003-2219-5833
dc.relation.departmentThe Linhardt Research Labs.
dc.relation.departmentThe Shirley Ann Jackson, Ph.D. Center for Biotechnology and Interdisciplinary Studies (CBIS)
rpi.description.pages11643-11648
rpi.description.volume3


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