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    Heparin/heparan sulfate analysis by covalently modified reverse polarity capillary zone electrophoresis-mass spectrometry

    Author
    Sanderson, Patience; Stickney, Morgan; Leach, Franklin E.; Xia, Qiangwei; Yu, Yanlei; Zhang, Fuming; Linhardt, Robert J.; Amster, I. Jonathan
    ORCID
    https://orcid.org/0000-0003-2219-5833
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    HEPARIN HEPARAN SULFATE ANALYSIS BY COVALENTLY MODIFIED.pdf (1.108Mb)
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    Date Issued
    2018-04-13
    Subject
    Biology; Chemistry and chemical biology; Chemical and biological engineering; Biomedical engineering
    Degree
    Terms of Use
    In Copyright : this Item is protected by copyright and/or related rights. You are free to use this Item in any way that is permitted by the copyright and related rights legislation that applies to your use. For other uses you need to obtain permission from the rights-holder(s). https://rightsstatements.org/page/InC/1.0/;
    Full Citation
    Heparin/heparan sulfate analysis by covalently modified reverse polarity capillary zone electrophoresis-mass spectrometry, P. Sanderson, M. Stickney, F. E. Leach III, Q. Xia, Y. Yu, F. Zhang R. J Linhardt, I. J. Amster, Journal of Chromatography A, 1545, 75–83, 2018.
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    URI
    https://hdl.handle.net/20.500.13015/5375; https://doi.org/10.1016/j.chroma.2018.02.052
    Abstract
    Reverse polarity capillary zone electrophoresis coupled to negative ion mode mass spectrometry (CZE-MS) is shown to be an effective and sensitive tool for the analysis of glycosaminoglycan mixtures. Covalent modification of the inner wall of the separation capillary with neutral or cationic reagents produces a stable and durable surface that provides reproducible separations. By combining CZE-MS with a cation-coated capillary and a sheath flow interface, a rapid and reliable method has been developed for the analysis of sulfated oligosaccharides from dp4 to dp12. Several different mixtures have been separated and detected by mass spectrometry. The mixtures were selected to test the capability of this approach to resolve subtle differences in structure, such as sulfation position and epimeric variation of the uronic acid. The system was applied to a complex mixture of heparin/heparan sulfate oligosaccharides varying in chain length from dp3 to dp12 and more than 80 molecular compositions were identified by accurate mass measurement.;
    Description
    Journal of Chromatography A, 1545, 75–83; Note : if this item contains full text it may be a preprint, author manuscript, or a Gold OA copy that permits redistribution with a license such as CC BY. The final version is available through the publisher’s platform.
    Department
    The Linhardt Research Labs.; The Shirley Ann Jackson, Ph.D. Center for Biotechnology and Interdisciplinary Studies (CBIS);
    Publisher
    Elsevier
    Relationships
    The Linhardt Research Labs Online Collection; Rensselaer Polytechnic Institute, Troy, NY; Journal of Chromatography A; https://harc.rpi.edu/;
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    A full text version is available in DSpace@RPI;
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