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    Novel Method for Measurement of Heparin Anticoagulant Activity Using SPR

    Author
    Zhao, Jing; Liu, Xinyue; Malhotra, Anju; Li, Quanhong; Zhang, Fuming; Linhardt, Robert J.
    ORCID
    https://orcid.org/0000-0003-2219-5833
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    NOVEL METHOD FOR MEASUREMENT OF HEPARIN ANTICOAGULANT.pdf (511.9Kb)
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    Date Issued
    2017-06-01
    Subject
    Biology; Chemistry and chemical biology; Chemical and biological engineering; Biomedical engineering
    Degree
    Terms of Use
    In Copyright : this Item is protected by copyright and/or related rights. You are free to use this Item in any way that is permitted by the copyright and related rights legislation that applies to your use. For other uses you need to obtain permission from the rights-holder(s). https://rightsstatements.org/page/InC/1.0/;
    Full Citation
    Novel Method for Measurement of Heparin Anticoagulant Activity Using SPR, J. Zhao, X. Liu, A. Malnotra, Q. Li, F. Zhang, R. J. Linhardt, Analytical Biochemistry, 526, 39-42, 2017.
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    URI
    https://hdl.handle.net/20.500.13015/5386; https://doi.org/10.1016/j.ab.2017.03.013
    Abstract
    A novel method has been developed for the easy measurement of heparin's anticoagulant activity using surface plasmon resonance. The anticoagulant activity of target heparin was evaluated by measuring the competitive antithrombin III binding of analyte heparin in the solution phase and USP heparin immobilized on chip surface. Heparins, obtained from different animal sources, and low molecular weight heparins were analyzed. The results were reproducible and correlated well with the results of chromogenic assays (correlation coefficient r = 0.98 for anti-Xa and r = 0.94 for anti-IIa). This protocol provides many advantages, significantly minimizing time, cost and the complications of chromogenic assay methods.;
    Description
    Analytical Biochemistry, 526, 39-42; Note : if this item contains full text it may be a preprint, author manuscript, or a Gold OA copy that permits redistribution with a license such as CC BY. The final version is available through the publisher’s platform.
    Department
    The Linhardt Research Labs.; The Shirley Ann Jackson, Ph.D. Center for Biotechnology and Interdisciplinary Studies (CBIS);
    Publisher
    Elsevier
    Relationships
    The Linhardt Research Labs Online Collection; Rensselaer Polytechnic Institute, Troy, NY; Analytical Biochemistry; https://harc.rpi.edu/;
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    A full text version is available in DSpace@RPI;
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