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dc.contributor.authorZhao, Jing
dc.contributor.authorLiu, Xinyue
dc.contributor.authorMalhotra, Anju
dc.contributor.authorLi, Quanhong
dc.contributor.authorZhang, Fuming
dc.contributor.authorLinhardt, Robert J.
dc.date2017
dc.date.accessioned2022-06-23T04:28:44Z
dc.date.available2022-06-23T04:28:44Z
dc.date.issued2017-06-01
dc.identifier.citationNovel Method for Measurement of Heparin Anticoagulant Activity Using SPR, J. Zhao, X. Liu, A. Malnotra, Q. Li, F. Zhang, R. J. Linhardt, Analytical Biochemistry, 526, 39-42, 2017.
dc.identifier.issn10960309
dc.identifier.issn32697
dc.identifier.urihttps://hdl.handle.net/20.500.13015/5386
dc.identifier.urihttps://doi.org/10.1016/j.ab.2017.03.013
dc.descriptionAnalytical Biochemistry, 526, 39-42
dc.descriptionNote : if this item contains full text it may be a preprint, author manuscript, or a Gold OA copy that permits redistribution with a license such as CC BY. The final version is available through the publisher’s platform.
dc.description.abstractA novel method has been developed for the easy measurement of heparin's anticoagulant activity using surface plasmon resonance. The anticoagulant activity of target heparin was evaluated by measuring the competitive antithrombin III binding of analyte heparin in the solution phase and USP heparin immobilized on chip surface. Heparins, obtained from different animal sources, and low molecular weight heparins were analyzed. The results were reproducible and correlated well with the results of chromogenic assays (correlation coefficient r = 0.98 for anti-Xa and r = 0.94 for anti-IIa). This protocol provides many advantages, significantly minimizing time, cost and the complications of chromogenic assay methods.
dc.description.sponsorshipNational Institutes of Health
dc.languageen_US
dc.language.isoENG
dc.publisherElsevier
dc.relation.ispartofThe Linhardt Research Labs Online Collection
dc.relation.ispartofRensselaer Polytechnic Institute, Troy, NY
dc.relation.ispartofAnalytical Biochemistry
dc.relation.urihttps://harc.rpi.edu/
dc.subjectBiology
dc.subjectChemistry and chemical biology
dc.subjectChemical and biological engineering
dc.subjectBiomedical engineering
dc.titleNovel Method for Measurement of Heparin Anticoagulant Activity Using SPR
dc.typeArticle
dcterms.accessRightsA full text version is available in DSpace@RPI
dcterms.isPartOfJournal
dcterms.isVersionOfhttps://doi.org/10.1016/j.ab.2017.03.013
dc.rights.holderIn Copyright : this Item is protected by copyright and/or related rights. You are free to use this Item in any way that is permitted by the copyright and related rights legislation that applies to your use. For other uses you need to obtain permission from the rights-holder(s). https://rightsstatements.org/page/InC/1.0/
dc.creator.identifierhttps://orcid.org/0000-0003-2219-5833
dc.relation.departmentThe Linhardt Research Labs.
dc.relation.departmentThe Shirley Ann Jackson, Ph.D. Center for Biotechnology and Interdisciplinary Studies (CBIS)
rpi.description.pages39-42
rpi.description.volume526


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