• Login
    View Item 
    •   DSpace@RPI Home
    • The Linhardt Research Labs
    • Linhardt Research Labs Papers
    • View Item
    •   DSpace@RPI Home
    • The Linhardt Research Labs
    • Linhardt Research Labs Papers
    • View Item
    JavaScript is disabled for your browser. Some features of this site may not work without it.

    Designer DNA architecture offers precise and multivalent spatial pattern-recognition for viral sensing and inhibition

    Author
    Kwon, Paul S.; Ren, Shaokang; Kwon, Seok Joon; Kizer, Megan E.; Kuo, Lili; Xie, Mo; Zhu, Dan; Zhou, Feng; Zhang, Fuming; Kim, Domyoung; Fraser, Keith; Kramer, Laura D.; Seeman, Nadrian C.; Dordick, Jonathan S.; Linhardt, Robert J.; Chao, Jie; Wang, Xing
    ORCID
    https://orcid.org/0000-0003-2219-5833
    Thumbnail
    View/Open
    Designer DNA architecture offers precise and multivalent spatial pattern-recognition for viral sensing and inhibition (1).pdf (3.197Mb)
    Other Contributors
    Date Issued
    2020-01-01
    Subject
    Biology; Chemistry and chemical biology; Chemical and biological engineering; Biomedical engineering
    Degree
    Terms of Use
    In Copyright : this Item is protected by copyright and/or related rights. You are free to use this Item in any way that is permitted by the copyright and related rights legislation that applies to your use. For other uses you need to obtain permission from the rights-holder(s). https://rightsstatements.org/page/InC/1.0/;
    Full Citation
    Designer DNA architecture offers precise and multivalent spatial pattern-recognition for viral sensing and inhibition, P. S. Kwon, S. Ren1, S.-J. Kwon, M. E. Kizer, L. Kuo, M. Xie, D. Zhu, F. Zhou, F. Zhang, D. Kim, K. Fraser, L. D. Kramer, N. C. Seeman, J. S. Dordick, R. J. Linhardt, J. Chao, X. Wang, Nature Chemistry, 12, 26–35, 2020.
    Metadata
    Show full item record
    URI
    https://hdl.handle.net/20.500.13015/5400; https://doi.org/10.1038/s41557-019-0369-8
    Abstract
    DNA, when folded into nanostructures with a specific shape, is capable of spacing and arranging binding sites into a complex geometric pattern with nanometre precision. Here we demonstrate a designer DNA nanostructure that can act as a template to display multiple binding motifs with precise spatial pattern-recognition properties, and that this approach can confer exceptional sensing and potent viral inhibitory capabilities. A star-shaped DNA architecture, carrying five molecular beacon-like motifs, was constructed to display ten dengue envelope protein domain III (ED3)-targeting aptamers into a two-dimensional pattern precisely matching the spatial arrangement of ED3 clusters on the dengue (DENV) viral surface. The resulting multivalent interactions provide high DENV-binding avidity. We show that this structure is a potent viral inhibitor and that it can act as a sensor by including a fluorescent output to report binding. Our molecular-platform design strategy could be adapted to detect and combat other disease-causing pathogens by generating the requisite ligand patterns on customized DNA nanoarchitectures.;
    Description
    Nature Chemistry, 12, 26–35; Note : if this item contains full text it may be a preprint, author manuscript, or a Gold OA copy that permits redistribution with a license such as CC BY. The final version is available through the publisher’s platform.
    Department
    The Linhardt Research Labs.; The Shirley Ann Jackson, Ph.D. Center for Biotechnology and Interdisciplinary Studies (CBIS);
    Publisher
    Nature
    Relationships
    The Linhardt Research Labs Online Collection; Rensselaer Polytechnic Institute, Troy, NY; Nature Chemistry; https://harc.rpi.edu/;
    Access
    Open Access; A full text version is available in DSpace@RPI;
    Collections
    • Linhardt Research Labs Papers

    Browse

    All of DSpace@RPICommunities & CollectionsBy Issue DateAuthorsTitlesSubjectsThis CollectionBy Issue DateAuthorsTitlesSubjects

    My Account

    Login

    DSpace software copyright © 2002-2022  DuraSpace
    Contact Us | Send Feedback
    DSpace Express is a service operated by 
    Atmire NV