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dc.rights.licenseOpen Access
dc.contributor.authorKwon, Paul S.
dc.contributor.authorRen, Shaokang
dc.contributor.authorKwon, Seok Joon
dc.contributor.authorKizer, Megan E.
dc.contributor.authorKuo, Lili
dc.contributor.authorXie, Mo
dc.contributor.authorZhu, Dan
dc.contributor.authorZhou, Feng
dc.contributor.authorZhang, Fuming
dc.contributor.authorKim, Domyoung
dc.contributor.authorFraser, Keith
dc.contributor.authorKramer, Laura D.
dc.contributor.authorSeeman, Nadrian C.
dc.contributor.authorDordick, Jonathan S.
dc.contributor.authorLinhardt, Robert J.
dc.contributor.authorChao, Jie
dc.contributor.authorWang, Xing
dc.date2020
dc.date.accessioned2022-06-23T04:45:59Z
dc.date.available2022-06-23T04:45:59Z
dc.date.issued2020-01-01
dc.identifier.citationDesigner DNA architecture offers precise and multivalent spatial pattern-recognition for viral sensing and inhibition, P. S. Kwon, S. Ren1, S.-J. Kwon, M. E. Kizer, L. Kuo, M. Xie, D. Zhu, F. Zhou, F. Zhang, D. Kim, K. Fraser, L. D. Kramer, N. C. Seeman, J. S. Dordick, R. J. Linhardt, J. Chao, X. Wang, Nature Chemistry, 12, 26–35, 2020.
dc.identifier.issn17554349
dc.identifier.issn17554330
dc.identifier.urihttps://hdl.handle.net/20.500.13015/5400
dc.identifier.urihttps://doi.org/10.1038/s41557-019-0369-8
dc.descriptionNature Chemistry, 12, 26–35
dc.descriptionNote : if this item contains full text it may be a preprint, author manuscript, or a Gold OA copy that permits redistribution with a license such as CC BY. The final version is available through the publisher’s platform.
dc.description.abstractDNA, when folded into nanostructures with a specific shape, is capable of spacing and arranging binding sites into a complex geometric pattern with nanometre precision. Here we demonstrate a designer DNA nanostructure that can act as a template to display multiple binding motifs with precise spatial pattern-recognition properties, and that this approach can confer exceptional sensing and potent viral inhibitory capabilities. A star-shaped DNA architecture, carrying five molecular beacon-like motifs, was constructed to display ten dengue envelope protein domain III (ED3)-targeting aptamers into a two-dimensional pattern precisely matching the spatial arrangement of ED3 clusters on the dengue (DENV) viral surface. The resulting multivalent interactions provide high DENV-binding avidity. We show that this structure is a potent viral inhibitor and that it can act as a sensor by including a fluorescent output to report binding. Our molecular-platform design strategy could be adapted to detect and combat other disease-causing pathogens by generating the requisite ligand patterns on customized DNA nanoarchitectures.
dc.description.sponsorshipNational Science Foundation
dc.languageen_US
dc.language.isoENG
dc.publisherNature
dc.relation.ispartofThe Linhardt Research Labs Online Collection
dc.relation.ispartofRensselaer Polytechnic Institute, Troy, NY
dc.relation.ispartofNature Chemistry
dc.relation.urihttps://harc.rpi.edu/
dc.subjectBiology
dc.subjectChemistry and chemical biology
dc.subjectChemical and biological engineering
dc.subjectBiomedical engineering
dc.titleDesigner DNA architecture offers precise and multivalent spatial pattern-recognition for viral sensing and inhibition
dc.typeArticle
dcterms.accessRightsA full text version is available in DSpace@RPI
dcterms.isPartOfJournal
dcterms.isVersionOfhttps://doi.org/10.1038/s41557-019-0369-8
dc.rights.holderIn Copyright : this Item is protected by copyright and/or related rights. You are free to use this Item in any way that is permitted by the copyright and related rights legislation that applies to your use. For other uses you need to obtain permission from the rights-holder(s). https://rightsstatements.org/page/InC/1.0/
dc.creator.identifierhttps://orcid.org/0000-0003-2219-5833
dc.relation.departmentThe Linhardt Research Labs.
dc.relation.departmentThe Shirley Ann Jackson, Ph.D. Center for Biotechnology and Interdisciplinary Studies (CBIS)
rpi.description.pages26-35
rpi.description.volume12


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