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dc.contributor.authorLariviere, Wells B.
dc.contributor.authorHan, Xiaorui
dc.contributor.authorOshima, Kaori
dc.contributor.authorMcMurtry, Sarah A.
dc.contributor.authorLinhardt, Robert J.
dc.contributor.authorSchmidt, Eric P.
dc.date2021
dc.date.accessioned2022-06-23T04:46:01Z
dc.date.available2022-06-23T04:46:01Z
dc.date.issued2021-02-01
dc.identifier.citationDetection of glycosaminoglycans by polyacrylamide gel electrophoresis and silver staining, W. B. LaRiviere, X. Han, K. Oshima, S. A. McMurtry, R. J. Linhardt, E. P. Schmidt, Journal of Visualized Experiments, 168, e62319, 2021.
dc.identifier.issn1940087X
dc.identifier.urihttps://hdl.handle.net/20.500.13015/5407
dc.identifier.urihttps://doi.org/10.3791/62319
dc.descriptionJournal of Visualized Experiments, 168, e62319
dc.descriptionNote : if this item contains full text it may be a preprint, author manuscript, or a Gold OA copy that permits redistribution with a license such as CC BY. The final version is available through the publisher’s platform.
dc.description.abstractSulfated glycosaminoglycans (GAGs) such as heparan sulfate (HS) and chondroitin sulfate (CS) are ubiquitous in living organisms and play a critical role in a variety of basic biological structures and processes. As polymers, GAGs exist as a polydisperse mixture containing polysaccharide chains that can range from 4000 Da to well over 40,000 Da. Within these chains exists domains of sulfation, conferring a pattern of negative charge that facilitates interaction with positively charged residues of cognate protein ligands. Sulfated domains of GAGs must be of sufficient length to allow for these electrostatic interactions. To understand the function of GAGs in biological tissues, the investigator must be able to isolate, purify, and measure the size of GAGs. This report describes a practical and versatile polyacrylamide gel electrophoresis-based technique that can be leveraged to resolve relatively small differences in size between GAGs isolated from a variety of biological tissue types.
dc.languageen_US
dc.language.isoENG
dc.publisherJove
dc.relation.ispartofThe Linhardt Research Labs Online Collection
dc.relation.ispartofRensselaer Polytechnic Institute, Troy, NY
dc.relation.ispartofJournal of Visualized Experiments
dc.relation.urihttps://harc.rpi.edu/
dc.subjectBiology
dc.subjectChemistry and chemical biology
dc.subjectChemical and biological engineering
dc.subjectBiomedical engineering
dc.titleDetection of glycosaminoglycans by polyacrylamide gel electrophoresis and silver staining
dc.typeArticle
dcterms.accessRightsA full text version is available in DSpace@RPI
dcterms.isPartOfJournal
dcterms.isVersionOfhttps://doi.org/10.3791/62319
dc.rights.holderIn Copyright : this Item is protected by copyright and/or related rights. You are free to use this Item in any way that is permitted by the copyright and related rights legislation that applies to your use. For other uses you need to obtain permission from the rights-holder(s). https://rightsstatements.org/page/InC/1.0/
dc.creator.identifierhttps://orcid.org/0000-0003-2219-5833
dc.relation.departmentThe Linhardt Research Labs.
dc.relation.departmentThe Shirley Ann Jackson, Ph.D. Center for Biotechnology and Interdisciplinary Studies (CBIS)
rpi.description.volume2021


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