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    The effect of electrospun scaffolds on the glycosaminoglycan profile of differentiating neural stem cells

    Author
    Garrudo, F.F.F.; Mikael, P.E.; Xia, K.; Silva, J.C.; Ouyang, Y.; Chapman, C.A.; Hoffman, P.R.; Yu, Y.; Han, X.; Rodrigues, C.A.V.; Cabral, J.M.S.; Morgado, J.; Ferreir F.C.; Linhardt, R. J.
    ORCID
    https://orcid.org/0000-0003-2219-5833
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    THE EFFECT OF ELECTROSPUN SCAFFOLDS ON THE.pdf (2.011Mb)
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    Date Issued
    2021-03-01
    Subject
    Biology; Chemistry and chemical biology; Chemical and biological engineering; Biomedical engineering
    Degree
    Terms of Use
    In Copyright : this Item is protected by copyright and/or related rights. You are free to use this Item in any way that is permitted by the copyright and related rights legislation that applies to your use. For other uses you need to obtain permission from the rights-holder(s). https://rightsstatements.org/page/InC/1.0/;
    Full Citation
    The effect of electrospun scaffolds on the glycosaminoglycan profile of differentiating neural stem cells, F. F. F. Garrudo, P. E. Mikael, K. Xia, J. C. Silva, Y. Ouyang, C. A. Chapman, P. R. Hoffman, Y. Yu, X. Han, C. A. V. Rodrigues, J. M. S. Cabral, J. Morgado, F. C. Ferreir R. J. Linhardt, Biochimie, 182, 61-72, 2021.
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    URI
    https://hdl.handle.net/20.500.13015/5410; https://doi.org/10.1016/j.biochi.2021.01.001
    Abstract
    The use of electrospun scaffolds for neural tissue engineering applications allows a closer mimicry of the native tissue extracellular matrix (ECM), important for the transplantation of cells in vivo. Moreover, the role of the electrospun fiber mat topography on neural stem cell (NSC) differentiation remains to be completely understood. In this work REN-VM cells (NSC model) were differentiated on polycaprolactone (PCL) nanofibers, obtained by wet/wet electrospinning, and on flat glass lamellas. The obtained differentiation profile of NSCs was evaluated using immunofluorescence and qPCR analysis. Glycosaminoglycan (GAG) analysis was successfully emplyed to evaluate changes in the GAG profile of differentiating cells through the use of the highly sensitive liquid chromatography-tandem mass/mass spectrometry (LC-MS/MS) method. Our results show that both culture platforms allow the differentiation of REN-VM cells into neural cells (neurons and astrocytes) similarly. Moreover, LC-MS/MS analysis shows changes in the production of GAGs present both in cell cultures and conditioned media samples. In the media, hyaluronic acid (HA) was detected and correlated with cellular activity and the production of a more plastic extracellular matrix. The cell samples evidence changes in chondroitin sulfate (CS4S, CS6S, CS4S6S) and heparan sulfate (HS6S, HS0S), similar to those previously described in vivo studies and possibly associated with the creation of complex structures, such as perineural networks. The GAG profile of differentiating REN-VM cells on electrospun scaffolds was analyzed for the first time. Our results highlight the advantage of using platforms obtain more reliable and robust neural tissue-engineered transplants.;
    Description
    Biochimie, 182, 61-72; Note : if this item contains full text it may be a preprint, author manuscript, or a Gold OA copy that permits redistribution with a license such as CC BY. The final version is available through the publisher’s platform.
    Department
    The Linhardt Research Labs.; The Shirley Ann Jackson, Ph.D. Center for Biotechnology and Interdisciplinary Studies (CBIS);
    Publisher
    Elsevier
    Relationships
    The Linhardt Research Labs Online Collection; Rensselaer Polytechnic Institute, Troy, NY; Biochimie; https://harc.rpi.edu/;
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    A full text version is available in DSpace@RPI;
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