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dc.contributor.authorWang, H.M.
dc.contributor.authorLoganathan, D.
dc.contributor.authorLinhardt, Robert J.
dc.date1991
dc.date.accessioned2022-06-27T15:33:48Z
dc.date.available2022-06-27T15:33:48Z
dc.date.issued1991
dc.identifier.citationDetermination of the pKa of Glucuronic Acid and Heparin's Carboxyl Groups by 13C NMR, H.M. Wang, D. Loganathan, R.J. Linhardt, Biochemical Journal, 278, 689-695 (1991).
dc.identifier.urihttps://doi.org/10.1042/bj2780689
dc.identifier.urihttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC1151401/https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1151401/
dc.identifier.urihttps://hdl.handle.net/20.500.13015/5420
dc.descriptionBiochemical Journal, 278, 689-695
dc.descriptionNote : if this item contains full text it may be a preprint, author manuscript, or a Gold OA copy that permits redistribution with a license such as CC BY. The final version is available through the publisher’s platform.
dc.description.abstractAs part of our continuing studies on heparin, the present paper uses 13C-n.m.r. spectroscopy to examine the acidity of heparin's uronic acid carboxylate groups. Heparin contains three different uronic acids. In porcine mucosal heparin these account for approx. 91, 7 and 2 mol% of the total uronic acid residues. These are alpha-L-idopyranosyluronic acid 2-sulphate, beta-D-glucopyranosyluronic acid and alpha-L-idopyranosyluronic acid. The pKa values of their carboxylate groups were determined as 3.13 (using heparin), 2.79 (using heparin) and 3.0 (predicted by using model compounds) respectively. 18C-n.m.r. spectroscopy, performed at various pH values, provided a convenient method of simultaneously determining the pKa of multiple carboxylate groups, of similar acidity, within heparin D-Glucopyranosyluronic acid and heparin-derived di-, tetra- and hexa-saccharides were used as model compounds to determine pKa values of the different carboxy groups. The results suggested that molecular size had an effect on pKa. Unambiguous assignment of carboxy carbon resonances were accomplished through the use of two-dimensional n.m.r. spectroscopy. Finally, application of this method to the simplest model compound, D-glucopyranosyluronic acid, permitted the determination of the pKa of both its alpha- and beta-anomers.
dc.description.urihttps://login.libproxy.rpi.edu/login?url=https://doi.org/10.1042/bj2780689
dc.description.urihttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC1151401/
dc.languageen_US
dc.language.isoENG
dc.relation.ispartofThe Linhardt Research Labs Online Collection
dc.relation.ispartofRensselaer Polytechnic Institute, Troy, NY
dc.relation.urihttps://harc.rpi.edu/
dc.subjectBiology
dc.subjectChemistry and chemical biology
dc.subjectChemical and biological engineering
dc.subjectBiomedical engineering
dc.titleDetermination of the pKa of Glucuronic Acid and Heparin's Carboxyl Groups by 13C NMR
dc.typeArticle
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dcterms.accessRightshttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC1151401/
dcterms.isVersionOfhttps://doi.org/10.1042/bj2780689
dc.rights.holderIn Copyright : this Item is protected by copyright and/or related rights. You are free to use this Item in any way that is permitted by the copyright and related rights legislation that applies to your use. For other uses you need to obtain permission from the rights-holder(s). https://rightsstatements.org/page/InC/1.0/
dc.creator.identifierhttps://orcid.org/0000-0003-2219-5833
dc.relation.departmentThe Linhardt Research Labs.
dc.relation.departmentThe Shirley Ann Jackson, Ph.D. Center for Biotechnology and Interdisciplinary Studies (CBIS)


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