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dc.contributor.authorXiao, Feng
dc.contributor.authorLian, Jiazhang
dc.contributor.authorTu, Shuai
dc.contributor.authorXie, Linlin
dc.contributor.authorLi, Jun
dc.contributor.authorZhang, Fuming
dc.contributor.authorLinhardt, Robert J.
dc.contributor.authorHuang, Haichan
dc.contributor.authorZhong, Weihong
dc.date2022
dc.date.accessioned2022-06-27T15:33:48Z
dc.date.available2022-06-27T15:33:48Z
dc.date.issued2022-02-18
dc.identifier.citationMetabolic Engineering of Saccharomyces cerevisiae for High-Level Production of Chlorogenic Acid from Glucose, F. Xiao, J. Lian, S. Tu, L. Xie, J. Li, F. Zhang, R. J. Linhardt, H. Huang, W. Zhong, ACS Synthetic Biology, 11, 800-811, 2022
dc.identifier.issn21615063
dc.identifier.urihttps://doi.org/10.1021/acssynbio.1c00487
dc.identifier.urihttps://hdl.handle.net/20.500.13015/5430
dc.descriptionACS Synthetic Biology, 11, 800-811
dc.descriptionNote : if this item contains full text it may be a preprint, author manuscript, or a Gold OA copy that permits redistribution with a license such as CC BY. The final version is available through the publisher’s platform.
dc.description.abstractChlorogenic acid (CGA), a major dietary phenolic compound, has been increasingly used in the food and pharmaceutical industries because of its ready availability and extensive biological and pharmacological activities. Traditionally, extraction from plants has been the main approach for the commercial production of CGA. This study reports the first efficient microbial production of CGA by engineering the yeast, Saccharomyces cerevisiae, on a simple mineral medium. First, an optimized de novo biosynthetic pathway for CGA was reconstructed in S. cerevisiae from glucose with a CGA titer of 36.6 ± 2.4 mg/L. Then, a multimodule engineering strategy was employed to improve CGA production: (1) unlocking the shikimate pathway and optimizing carbon distribution; (2) optimizing the l-Phe branch and pathway balancing; and (3) increasing the copy number of CGA pathway genes. The combination of these interventions resulted in an about 6.4-fold improvement of CGA titer up to 234.8 ± 11.1 mg/L in shake flask cultures. CGA titers of 806.8 ± 1.7 mg/L were achieved in a 1 L fed-batch fermenter. This study opens a route to effectively produce CGA from glucose in S. cerevisiae and establishes a platform for the biosynthesis of CGA-derived value-added metabolites.
dc.description.sponsorshipNational Natural Science Foundation of China
dc.description.urihttps://login.libproxy.rpi.edu/login?url=https://doi.org/10.1021/acssynbio.1c00487
dc.languageen_US
dc.language.isoENG
dc.relation.ispartofThe Linhardt Research Labs Online Collection
dc.relation.ispartofRensselaer Polytechnic Institute, Troy, NY
dc.relation.ispartofACS Synthetic Biology
dc.relation.urihttps://harc.rpi.edu/
dc.subjectBiology
dc.subjectChemistry and chemical biology
dc.subjectChemical and biological engineering
dc.subjectBiomedical engineering
dc.titleMetabolic Engineering of Saccharomyces cerevisiae for High-Level Production of Chlorogenic Acid from Glucose
dc.typeArticle
dcterms.accessRightshttps://login.libproxy.rpi.edu/login?url=https://doi.org/10.1021/acssynbio.1c00487
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dcterms.isVersionOfhttps://doi.org/10.1021/acssynbio.1c00487
dc.rights.holderIn Copyright : this Item is protected by copyright and/or related rights. You are free to use this Item in any way that is permitted by the copyright and related rights legislation that applies to your use. For other uses you need to obtain permission from the rights-holder(s). https://rightsstatements.org/page/InC/1.0/
dc.creator.identifierhttps://orcid.org/0000-0003-2219-5833
dc.relation.departmentThe Linhardt Research Labs.
dc.relation.departmentThe Shirley Ann Jackson, Ph.D. Center for Biotechnology and Interdisciplinary Studies (CBIS)
rpi.description.pages800-811
rpi.description.volume11


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