| dc.contributor.author | Fu, Li | |
| dc.contributor.author | Linhardt, Robert J. | |
| dc.date | 2022 | |
| dc.date.accessioned | 2022-06-27T15:36:07Z | |
| dc.date.available | 2022-06-27T15:36:07Z | |
| dc.date.issued | 2022-01-01 | |
| dc.identifier.citation | One-Pot Enzymatic Synthesis of Heparin from N-Sulfoheparosan, L. Fu, R. J. Linhardt, Methods in Molecular Biology, 2303, 3-11, 2022. | |
| dc.identifier.issn | 19406029 | |
| dc.identifier.issn | 10643745 | |
| dc.identifier.uri | https://doi.org/10.1007/978-1-0716-1398-6_1 | |
| dc.identifier.uri | https://hdl.handle.net/20.500.13015/5436 | |
| dc.description | Methods in Molecular Biology, 2303, 3-11 | |
| dc.description | Note : if this item contains full text it may be a preprint, author manuscript, or a Gold OA copy that permits redistribution with a license such as CC BY. The final version is available through the publisher’s platform. | |
| dc.description.abstract | Heparin, a glycosaminoglycan-based anticoagulant drug, is prepared as an extract of animal tissues. Heparosan, an Escherichia coli (E. coli) K5 capsular polysaccharide with the structure →4)-β-D-glucuronic acid (1 → 4)-β-D-N-acetylglucosamine (1→, corresponds to the precursor backbone in the Golgi-based biosynthesis of heparin. Anticoagulant heparin is prepared in a one-pot synthesis using a chemically prepared derivative of heparosan called N-sulfoheparosan (NSH), recombinant Golgi enzymes expressed in E. coli, and the 3-phosphoadenosine-5-phosphosulfate (PAPS) cofactor. | |
| dc.description.sponsorship | National Institutes of Health | |
| dc.description.uri | https://login.libproxy.rpi.edu/login?url=https://doi.org/10.1007/978-1-0716-1398-6_1 | |
| dc.language | en_US | |
| dc.language.iso | ENG | |
| dc.relation.ispartof | The Linhardt Research Labs Online Collection | |
| dc.relation.ispartof | Rensselaer Polytechnic Institute, Troy, NY | |
| dc.relation.ispartof | Methods in Molecular Biology | |
| dc.relation.uri | https://harc.rpi.edu/ | |
| dc.subject | Biology | |
| dc.subject | Chemistry and chemical biology | |
| dc.subject | Chemical and biological engineering | |
| dc.subject | Biomedical engineering | |
| dc.title | One-Pot Enzymatic Synthesis of Heparin from N-Sulfoheparosan | |
| dc.type | Article | |
| dcterms.accessRights | https://login.libproxy.rpi.edu/login?url=https://doi.org/10.1007/978-1-0716-1398-6_1 | |
| dcterms.isPartOf | Book Series | |
| dcterms.isVersionOf | https://doi.org/10.1007/978-1-0716-1398-6_1 | |
| dc.rights.holder | In Copyright : this Item is protected by copyright and/or related rights. You are free to use this Item in any way that is permitted by the copyright and related rights legislation that applies to your use. For other uses you need to obtain permission from the rights-holder(s). https://rightsstatements.org/page/InC/1.0/ | |
| dc.creator.identifier | https://orcid.org/0000-0003-2219-5833 | |
| dc.relation.department | The Linhardt Research Labs. | |
| dc.relation.department | The Shirley Ann Jackson, Ph.D. Center for Biotechnology and Interdisciplinary Studies (CBIS) | |
| rpi.description.pages | 3-11 | |
| rpi.description.volume | 2303 | |
