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dc.contributor.authorFu, Li
dc.contributor.authorLinhardt, Robert J.
dc.date2022
dc.date.accessioned2022-06-27T15:36:07Z
dc.date.available2022-06-27T15:36:07Z
dc.date.issued2022-01-01
dc.identifier.citationOne-Pot Enzymatic Synthesis of Heparin from N-Sulfoheparosan, L. Fu, R. J. Linhardt, Methods in Molecular Biology, 2303, 3-11, 2022.
dc.identifier.issn19406029
dc.identifier.issn10643745
dc.identifier.urihttps://doi.org/10.1007/978-1-0716-1398-6_1
dc.identifier.urihttps://hdl.handle.net/20.500.13015/5436
dc.descriptionMethods in Molecular Biology, 2303, 3-11
dc.descriptionNote : if this item contains full text it may be a preprint, author manuscript, or a Gold OA copy that permits redistribution with a license such as CC BY. The final version is available through the publisher’s platform.
dc.description.abstractHeparin, a glycosaminoglycan-based anticoagulant drug, is prepared as an extract of animal tissues. Heparosan, an Escherichia coli (E. coli) K5 capsular polysaccharide with the structure →4)-β-D-glucuronic acid (1 → 4)-β-D-N-acetylglucosamine (1→, corresponds to the precursor backbone in the Golgi-based biosynthesis of heparin. Anticoagulant heparin is prepared in a one-pot synthesis using a chemically prepared derivative of heparosan called N-sulfoheparosan (NSH), recombinant Golgi enzymes expressed in E. coli, and the 3-phosphoadenosine-5-phosphosulfate (PAPS) cofactor.
dc.description.sponsorshipNational Institutes of Health
dc.description.urihttps://login.libproxy.rpi.edu/login?url=https://doi.org/10.1007/978-1-0716-1398-6_1
dc.languageen_US
dc.language.isoENG
dc.relation.ispartofThe Linhardt Research Labs Online Collection
dc.relation.ispartofRensselaer Polytechnic Institute, Troy, NY
dc.relation.ispartofMethods in Molecular Biology
dc.relation.urihttps://harc.rpi.edu/
dc.subjectBiology
dc.subjectChemistry and chemical biology
dc.subjectChemical and biological engineering
dc.subjectBiomedical engineering
dc.titleOne-Pot Enzymatic Synthesis of Heparin from N-Sulfoheparosan
dc.typeArticle
dcterms.accessRightshttps://login.libproxy.rpi.edu/login?url=https://doi.org/10.1007/978-1-0716-1398-6_1
dcterms.isPartOfBook Series
dcterms.isVersionOfhttps://doi.org/10.1007/978-1-0716-1398-6_1
dc.rights.holderIn Copyright : this Item is protected by copyright and/or related rights. You are free to use this Item in any way that is permitted by the copyright and related rights legislation that applies to your use. For other uses you need to obtain permission from the rights-holder(s). https://rightsstatements.org/page/InC/1.0/
dc.creator.identifierhttps://orcid.org/0000-0003-2219-5833
dc.relation.departmentThe Linhardt Research Labs.
dc.relation.departmentThe Shirley Ann Jackson, Ph.D. Center for Biotechnology and Interdisciplinary Studies (CBIS)
rpi.description.pages3-11
rpi.description.volume2303


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