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dc.contributor.authorMietzsch, Mario
dc.contributor.authorYu, Jennifer C.
dc.contributor.authorHsi, Jane
dc.contributor.authorChipman, Paul
dc.contributor.authorBroecker, Felix
dc.contributor.authorFuming, Zhang
dc.contributor.authorLinhardt, Robert J.
dc.contributor.authorSeeberger, Peter H.
dc.contributor.authorHeilbronn, Regine
dc.contributor.authorMcKenna, Robert
dc.contributor.authorAgbandje-McKenna, Mavis
dc.date2021
dc.date.accessioned2022-06-27T15:36:07Z
dc.date.available2022-06-27T15:36:07Z
dc.date.issued2021-12-01
dc.identifier.citationStructural study of AAVrh.10 Receptor and Antibody Interactions, M. Mietzsch, J.C. Yu, J. Hsi, P. Chipman, F. Broecker, F. Zhang, R.J. Linhardt, P.H. Seeberger, R. Heilbronn, R. McKenna, M. Agbandje-McKenna. Journal of Virology, 22, JVI0124921, 2021.
dc.identifier.issn10985514
dc.identifier.issn0022538X
dc.identifier.urihttps://doi.org/10.1128/JVI.01249-21
dc.identifier.urihttps://hdl.handle.net/20.500.13015/5438
dc.descriptionJournal of Virology, 22, JVI0124921
dc.descriptionNote : if this item contains full text it may be a preprint, author manuscript, or a Gold OA copy that permits redistribution with a license such as CC BY. The final version is available through the publisher’s platform.
dc.description.abstractRecombinant adeno-associated virus (rAAV) vectors are one of the leading tools for the delivery of therapeutic genes in human gene therapy applications. For a successful transfer of their payload, the AAV vectors have to circumvent potential preexisting neutralizing host antibodies and bind to the receptors of the target cells. Both of these aspects have not been structurally analyzed for AAVrh.10. Here, cryo-electron microscopy and three-dimensional image reconstruction were used to map the binding site of sulfated N-acetyllactosamine (LacNAc; previously shown to bind AAVrh.10) and a series of four monoclonal antibodies (MAbs). LacNAc was found to bind to a pocket located on the side of the 3-fold capsid protrusion that is mostly conserved to AAV9 and equivalent to its galactose-binding site. As a result, AAVrh.10 was also shown to be able to bind to cell surface glycans with terminal galactose. For the antigenic characterization, it was observed that several anti-AAV8 MAbs cross-react with AAVrh.10. The binding sites of these antibodies were mapped to the 3-fold capsid protrusions. Based on these observations, the AAVrh.10 capsid surface was engineered to create variant capsids that escape these antibodies while maintaining infectivity. IMPORTANCE Gene therapy vectors based on adeno-associated virus rhesus isolate 10 (AAVrh.10) have been used in several clinical trials to treat monogenetic diseases. However, compared to other AAV serotypes little is known about receptor binding and antigenicity of the AAVrh.10 capsid. Particularly, preexisting neutralizing antibodies against capsids are an important challenge that can hamper treatment efficiency. This study addresses both topics and identifies critical regions of the AAVrh.10 capsid for receptor and antibody binding. The insights gained were utilized to generate AAVrh.10 variants capable of evading known neutralizing antibodies. The findings of this study could further aid the utilization of AAVrh.10 vectors in clinical trials and help the approval of the subsequent biologics.
dc.description.sponsorshipNational Institutes of Health
dc.languageen_US
dc.language.isoENG
dc.relation.ispartofThe Linhardt Research Labs Online Collection
dc.relation.ispartofRensselaer Polytechnic Institute, Troy, NY
dc.relation.ispartofJournal of Virology
dc.relation.urihttps://harc.rpi.edu/
dc.subjectBiology
dc.subjectChemistry and chemical biology
dc.subjectChemical and biological engineering
dc.subjectBiomedical engineering
dc.titleStructural study of AAVrh.10 Receptor and Antibody Interactions
dc.typeArticle
dcterms.isPartOfJournal
dcterms.isVersionOfhttps://doi.org/10.1128/JVI.01249-21
dc.rights.holderIn Copyright : this Item is protected by copyright and/or related rights. You are free to use this Item in any way that is permitted by the copyright and related rights legislation that applies to your use. For other uses you need to obtain permission from the rights-holder(s). https://rightsstatements.org/page/InC/1.0/
dc.creator.identifierhttps://orcid.org/0000-0003-2219-5833
dc.relation.departmentThe Linhardt Research Labs.
dc.relation.departmentThe Shirley Ann Jackson, Ph.D. Center for Biotechnology and Interdisciplinary Studies (CBIS)
rpi.description.volume95


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