dc.contributor.author | Zhang, Wenjing | |
dc.contributor.author | Wu, Wanli | |
dc.contributor.author | Bao, Yizhong | |
dc.contributor.author | Yan, Xiaojun | |
dc.contributor.author | Zhang, Fuming | |
dc.contributor.author | Linhardt, Robert J. | |
dc.contributor.author | Jin, Weihua | |
dc.contributor.author | Mao, Genxiang | |
dc.date | 2021 | |
dc.date.accessioned | 2022-06-27T15:36:07Z | |
dc.date.available | 2022-06-27T15:36:07Z | |
dc.date.issued | 2021-11-07 | |
dc.identifier.citation | Comparative study on the mechanisms of anti-lung cancer activities of three sulfated galactofucans, W. Zhang, W. Wu, Y. Bao, X. Yan, F. Zhang, R.J. Linhardt, W. Jin, G. Mao, Food and Function, 10644-10657, 2021. | |
dc.identifier.issn | 2042650X | |
dc.identifier.issn | 20426496 | |
dc.identifier.uri | https://doi.org/10.1039/d1fo02062e | |
dc.identifier.uri | https://hdl.handle.net/20.500.13015/5439 | |
dc.description | Food and Function, 10644-10657 | |
dc.description | Note : if this item contains full text it may be a preprint, author manuscript, or a Gold OA copy that permits redistribution with a license such as CC BY. The final version is available through the publisher’s platform. | |
dc.description.abstract | Sulfated galactofucans, as the active compositions of fucoidan, were reported to exhibit antitumor activity. In the current study, a sulfated galactofucan (SGF) from Sargassum thunbergii and its three derivatives (SGF-H, SGF-L, and SGF-S) were prepared for structural analysis. Structural analysis showed that SGF-H was a high molecular weight sulfated galactofucan (51.5/17.8 kDa) with a high molar ratio of galactose (Gal) to fucose (Fuc) (0.66 : 1), SGF-L was a low molecular weight sulfated galactofucan (17.7 kDa) with a low molar ratio of Gal to Fuc (0.20 : 1), and SGF-S was a mixture (1.7 kDa) of sulfated galacto-fuco-oligomers or fuco-oligomers. It was noteworthy that the linkage of Gal residues in SGF-H was a β-linkage while SGF-L was an α-linkage. A comparative study on the anti-lung cancer activity in vitro and in vivo, antimetastatic effects, the metastasis-associated protein expression, and binding abilities to fibroblast growth factors (FGFs) of SGF, SGF-H, and SGF-L was performed to understand the structure–activity relationship. To some extent, SGF-L showed the strongest activity in the inhibition of human lung cancer cells A549 cell proliferation, while SGF-H exhibited the strongest activity in the inhibition of human bronchial epithelial cells BEAS-2B cell proliferation. SGF-L showed the strongest antimetastatic activity, followed by SGF-H and SGF. The expression of metastasis-associated proteins showed only a small difference. The in vivo tumor inhibition of SGF, SGF-H, and SGF-L was 45%, 41%, and 31%, respectively. SPR analysis showed SGF-H binds preferentially to FGF1 and FGF2, while SGF-L preferentially binds to FGF7 and FGF10, suggesting that the anti-lung cancer activity from sulfated galactofucan could involve the FGF-FAK/mTOR pathway. | |
dc.description.sponsorship | National Institutes of Health | |
dc.description.uri | https://login.libproxy.rpi.edu/login?url=https://doi.org/10.1039/d1fo02062e | |
dc.language | en_US | |
dc.language.iso | ENG | |
dc.relation.ispartof | The Linhardt Research Labs Online Collection | |
dc.relation.ispartof | Rensselaer Polytechnic Institute, Troy, NY | |
dc.relation.ispartof | Food and Function | |
dc.relation.uri | https://harc.rpi.edu/ | |
dc.subject | Biology | |
dc.subject | Chemistry and chemical biology | |
dc.subject | Chemical and biological engineering | |
dc.subject | Biomedical engineering | |
dc.title | Comparative study on the mechanisms of anti-lung cancer activities of three sulfated galactofucans | |
dc.type | Article | |
dcterms.accessRights | https://login.libproxy.rpi.edu/login?url=https://doi.org/10.1039/d1fo02062e | |
dcterms.isPartOf | Journal | |
dcterms.isVersionOf | https://doi.org/10.1039/d1fo02062e | |
dc.rights.holder | In Copyright : this Item is protected by copyright and/or related rights. You are free to use this Item in any way that is permitted by the copyright and related rights legislation that applies to your use. For other uses you need to obtain permission from the rights-holder(s). https://rightsstatements.org/page/InC/1.0/ | |
dc.creator.identifier | https://orcid.org/0000-0003-2219-5833 | |
dc.relation.department | The Linhardt Research Labs. | |
dc.relation.department | The Shirley Ann Jackson, Ph.D. Center for Biotechnology and Interdisciplinary Studies (CBIS) | |
rpi.description.pages | 10644-10657 | |
rpi.description.volume | 12 | |