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    Anti-inflammation effects of highly purified low-Mw RG-I pectins on LPS-activated macrophages

    Author
    Wu, Dongmei; Zheng, Xiaoliang; Hu, Weiwei; Zhu, Kai; Yu, Chengxiao; He, Qiaojun; Linhardt, Robert J.; Ye, Xingqian; Chen, Shiguo
    ORCID
    https://orcid.org/0000-0003-2219-5833
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    Other Contributors
    Date Issued
    2021-11-01
    Subject
    Biology; Chemistry and chemical biology; Chemical and biological engineering; Biomedical engineering
    Degree
    Terms of Use
    In Copyright : this Item is protected by copyright and/or related rights. You are free to use this Item in any way that is permitted by the copyright and related rights legislation that applies to your use. For other uses you need to obtain permission from the rights-holder(s). https://rightsstatements.org/page/InC/1.0/;
    Full Citation
    Anti-inflammation effects of highly purified low-Mw RG-I pectins on LPS-activated macrophages, D. Wu, X. Zheng, W. Hu, K. Zhu, C. Yu, Q. He, R. J. Linhardt, X. Ye, S. Chen, Bioactive Carbohydrates and Dietary Fibre, 26, 100283, 2021.
    Metadata
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    URI
    https://doi.org/10.1016/j.bcdf.2021.100283; https://hdl.handle.net/20.500.13015/5441
    Abstract
    Pectic oligosaccharides are viewed as excellent immunomodulator, but the specific molecular weight (Mw) range required for anti-inflammatory effects remains unclear. In this study, citrus pectins was depolymerized by the ultrasound/H2O2/Vc system and the pectic mixtures was separated through the superdex 30 chromatography column. Three highly purified pectin fractions (USP1, USP2, USP3) were obtained, with the Mw of 2713 g/mol, 3683 g/mol, and 7469 g/mol. All of them were RG-I pectins and USP3 contained more arabinan sidechains than USP1 and USP2. In vitro anti-inflammation assays based on LPS-induced RAW264.7 macrophages indicated that these pectins could suppress the NO release, ROS production and down-regulated the level of pro-inflammatory cytokines IL-1β, IL-6 and TNF-α in a Mw-dependent manner. The inhibitory effects increased in the order of USP1<USP2<USP3. Meanwhile, the regulation of pro-inflammatory cytokines were closely related to the content of arabinan sidechains in pectin. Thus, low-Mw pectins with Mw>3KD should be effective as a prophylactic agent against inflammatory diseases in functional food.;
    Description
    Bioactive Carbohydrates and Dietary Fibre, 26, 100283; Note : if this item contains full text it may be a preprint, author manuscript, or a Gold OA copy that permits redistribution with a license such as CC BY. The final version is available through the publisher’s platform.
    Department
    The Linhardt Research Labs.; The Shirley Ann Jackson, Ph.D. Center for Biotechnology and Interdisciplinary Studies (CBIS);
    Relationships
    The Linhardt Research Labs Online Collection; Rensselaer Polytechnic Institute, Troy, NY; Bioactive Carbohydrates and Dietary Fibre; https://harc.rpi.edu/;
    Access
    https://login.libproxy.rpi.edu/login?url=https://doi.org/10.1016/j.bcdf.2021.100283;
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