Biology; Chemistry and chemical biology; Chemical and biological engineering; Biomedical engineering
In Copyright : this Item is protected by copyright and/or related rights. You are free to use this Item in any way that is permitted by the copyright and related rights legislation that applies to your use. For other uses you need to obtain permission from the rights-holder(s). https://rightsstatements.org/page/InC/1.0/;
Detection of Protamine Using a Promising Metal Organic Frameworks Based Fluorescent 1 Molecular Device BZA-BOD@ZIF-90, L. Liu, J. Dai, B. Shen, X. Zhang, R. J. Linhardt, Sensors and Actuators B: Chemical, 341, 130006, 2021.
Herein, we designed a BODIPY-based probe ([email protected]) with good stability through the encapsulation of metal-organic frameworks (MOFs). [email protected] can selectively detect protamine based on an aggregation-induced emission (AIE) effect. In the present strategy, the designed [email protected] showed excellent fluorescence response to protamine with a low detection limit of 0.07 µg/mL and the detection was not disturbed by other possible competing substances. Compared with previous methods, BZA-BOD designed by this method is simpler to obtain, and also can achieve sensitive and accurate determination of protamine. Subsequently, the nanocomposite BZA-BOD[email protected] was successfully applied to detect protamine spiked in human serum. In addition, due to the strong binding effect of heparin on protamine, when heparin was added to the complex, the fluorescence intensity of the [email protected] weakened, thus, it can also be utilized for heparin detection. The medical application of protamine and heparin suggests that this fluorescent molecular device has prospects for certain clinical applications.;
Sensors and Actuators B: Chemical, 341, 130006; Note : if this item contains full text it may be a preprint, author manuscript, or a Gold OA copy that permits redistribution with a license such as CC BY. The final version is available through the publisher’s platform.
The Linhardt Research Labs.; The Shirley Ann Jackson, Ph.D. Center for Biotechnology and Interdisciplinary Studies (CBIS);
The Linhardt Research Labs Online Collection; Rensselaer Polytechnic Institute, Troy, NY; https://harc.rpi.edu/;