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dc.contributor.authorLiu, Tengfei
dc.contributor.authorLi, Jun
dc.contributor.authorQiu, Lequan
dc.contributor.authorZhang, Fuming
dc.contributor.authorLinhardt, Robert J.
dc.contributor.authorZhong, Weihong
dc.date2020
dc.date.accessioned2022-06-27T15:38:39Z
dc.date.available2022-06-27T15:38:39Z
dc.date.issued2020-12-01
dc.identifier.citationCombined genomic and transcriptomic analysis of Dibutyl phthalate metabolic pathway in Arthrobacter sp. ZJUTW, T. Liu, J. Li, L. Qiu, F. Zhang, R. J. Linhardt, W. Zhong, Biotechnology and Bioengineering, 117, 3712–3726. 2020.
dc.identifier.issn10970290
dc.identifier.issn63592
dc.identifier.urihttps://doi.org/10.1002/bit.27524
dc.identifier.urihttps://hdl.handle.net/20.500.13015/5474
dc.descriptionBiotechnology and Bioengineering, 117, 3712–3726
dc.descriptionNote : if this item contains full text it may be a preprint, author manuscript, or a Gold OA copy that permits redistribution with a license such as CC BY. The final version is available through the publisher’s platform.
dc.description.abstractDibutyl phthalate (DBP) is an environmental pollutant that can threaten human health. The strain Arthrobacter sp. ZJUTW, isolated from the sludge of a river of Hangzhou, can efficiently degrade DBP. Its genomic and transcriptomic differences when cultivated with DBP compared with glucose revealed specific DBP metabolic pathways in the ZJUTW strain. The degrading gene clusters localize separately on a circular chromosome and a plasmid pQL1. Genes related to the initial steps of DBP degradation from DBP to phthalic acid (PA), the pehA gene, and pht gene cluster, are located on the plasmid pQL1. However, the pca gene cluster related to the transforming of intermediate protocatechuic acid (PCA) to acetyl-CoA, is located on the chromosome. After comparative analysis with the reported gene clusters, we found that there were a series of homologous genes in pht and pca gene clusters that contribute to the efficient degradation of DBP by ZJUTW. In addition, transcriptomic analysis suggested a synergistic effect between pht and pca clusters, which also favor ZJUTW allowing it to efficiently degrade DBP. Combined genomic and transcriptomic analyses revealed a complete DBP metabolic pathway in Arthrobacter sp. ZJUTW that is different from that of other reported Arthrobacter strains. After necessary modification based on its metabolic characteristics, Arthrobacter sp. ZJUTW or its derivatives might represent promising candidates for the bioremediation of DBP pollution.
dc.description.sponsorshipNatural Science Foundation of Zhejiang Province
dc.description.urihttps://login.libproxy.rpi.edu/login?url=https://doi.org/10.1002/bit.27524
dc.languageen_US
dc.language.isoENG
dc.relation.ispartofThe Linhardt Research Labs Online Collection
dc.relation.ispartofRensselaer Polytechnic Institute, Troy, NY
dc.relation.ispartofBiotechnology and Bioengineering
dc.relation.urihttps://harc.rpi.edu/
dc.subjectBiology
dc.subjectChemistry and chemical biology
dc.subjectChemical and biological engineering
dc.subjectBiomedical engineering
dc.titleCombined genomic and transcriptomic analysis of Dibutyl phthalate metabolic pathway in Arthrobacter sp. ZJUTW
dc.typeArticle
dcterms.accessRightshttps://login.libproxy.rpi.edu/login?url=https://doi.org/10.1002/bit.27524
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dcterms.isVersionOfhttps://doi.org/10.1002/bit.27524
dc.rights.holderIn Copyright : this Item is protected by copyright and/or related rights. You are free to use this Item in any way that is permitted by the copyright and related rights legislation that applies to your use. For other uses you need to obtain permission from the rights-holder(s). https://rightsstatements.org/page/InC/1.0/
dc.creator.identifierhttps://orcid.org/0000-0003-2219-5833
dc.relation.departmentThe Linhardt Research Labs.
dc.relation.departmentThe Shirley Ann Jackson, Ph.D. Center for Biotechnology and Interdisciplinary Studies (CBIS)
rpi.description.pages3712-3726
rpi.description.volume117


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