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dc.contributor.authorLiu, Xiuxia
dc.contributor.authorZhao, Zihao
dc.contributor.authorDong, Guibin
dc.contributor.authorLi, Ye
dc.contributor.authorPeng, Feng
dc.contributor.authorLiu, Chunli
dc.contributor.authorZhang, Fuming
dc.contributor.authorLinhardt, Robert J.
dc.contributor.authorYang, Yankun
dc.contributor.authorBai, Zhonghu
dc.identifier.citationIdentification, Repair and Characterization of a Benzyl Alcohol-Inducible Promoter for Recombinant Proteins Overexpression in Corynebacterium glutamicum, X. Liu, Z. Zhao, G. Dong, Y. Li, F. Peng, C. Liu, F. Zhang, R. J. Linhardt, Y. Yang, Z. Bai, Enzyme and Microbial Technology,141, 109651, 2020.
dc.descriptionEnzyme and Microbial Technology,141, 109651
dc.descriptionNote : if this item contains full text it may be a preprint, author manuscript, or a Gold OA copy that permits redistribution with a license such as CC BY. The final version is available through the publisher’s platform.
dc.description.abstractCorynebacterium glutamicum is an important industrial organism for the production of a variety of biological commodities. We discovered a promoter encoded by the gene NCgl2319 in C. glutamicum, which could be induced by benzyl alcohol, could be used as an efficient tunable expression system. In initial attempts, this promoter failed to function in a recombinant expression system. This was remedied by extending the original genetic context of the promoter, generating a new version Pcat-B. The Pcat-B transcription initiation site, its critical active regions, and its effect of inducers were fully characterized resulting in tunable expression. This approach proved to be very efficient in producing a pharmaceutical protein, N-terminal pro-brain natriuretic peptide (NT-proBNP). Production of approximately 440.43 mg/L NT-proBNP was achieved with the Pcat-B expression system demonstrating its application for controllable pharmaceutical protein production in C. glutamicum.
dc.description.sponsorshipNational Natural Science Foundation of China
dc.relation.ispartofThe Linhardt Research Labs Online Collection
dc.relation.ispartofRensselaer Polytechnic Institute, Troy, NY
dc.relation.ispartofEnzyme and Microbial Technology
dc.subjectChemistry and chemical biology
dc.subjectChemical and biological engineering
dc.subjectBiomedical engineering
dc.titleIdentification, Repair and Characterization of a Benzyl Alcohol-Inducible Promoter for Recombinant Proteins Overexpression in Corynebacterium glutamicum
dc.rights.holderIn Copyright : this Item is protected by copyright and/or related rights. You are free to use this Item in any way that is permitted by the copyright and related rights legislation that applies to your use. For other uses you need to obtain permission from the rights-holder(s).
dc.relation.departmentThe Linhardt Research Labs.
dc.relation.departmentThe Shirley Ann Jackson, Ph.D. Center for Biotechnology and Interdisciplinary Studies (CBIS)

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