Biology; Chemistry and chemical biology; Chemical and biological engineering; Biomedical engineering
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Glycocalyx-like hydrogel coatings for small diameter vascular grafts, S. Dimitrievska, J. Wang, T. Lin, A. Weyers, H. Bai, L. Qin, G. Li, C. Cai, A. Kypson, N. Kristofik, A. Gard, S. Sundaram, K. Yamamoto, W. Wu, L. Zhao, M. H. Kural, Y. Yuan, J. Madri, T. R. Kyriakides, R. J. Linhardt, L. E. Niklason, Advanced Functional Matererials, 30, 1908963, 2020.
Novel biological vascular conduits, such as decellularized tissue engineered vascular grafts (TEVGs) are hindered by high thrombogenicity. To mimic the antithrombogenic surface of native vessels with a continuous glycosaminoglycan layer that is present on endothelial cells (ECs), a hyaluronic acid (HA) modified surface is established, to effectively shield blood platelets from collagen-triggered activation. Using the amine groups present on 4 mm diameter decellularized TEVGs, a continuous HA hydrogel coating is built via a bifunctional thiol-reactive cross-linker, thereby avoiding nonspecific collagen matrix cross-linking. The HA hydrogel layer recreates a luminal wall, “hiding” exposed collagen from the bloodstream. In vitro blood tests show that adhered platelets, fibrinogen absorption, and fibrin formation on HA-coated decellularized TEVGs are significantly lower than on uncoated decellularized TEVGs. The HA surface also inhibits macrophage adhesion in vitro. HA-coated decellularized syngeneic rat aortae (≈1.5 mm diameter), and TEVGs in rat and canine models, respectively, are protected from aggressive thrombus formation, and preserve normal blood flow. Re-endothelialization is also observed. HA-coated TEVGs may be an off-the-shelf small-diameter vascular graft with dual benefits: antithrombogenic protection and promotion of endothelium.;
Advanced Functional Matererials, 30, 1908963; Note : if this item contains full text it may be a preprint, author manuscript, or a Gold OA copy that permits redistribution with a license such as CC BY. The final version is available through the publisher’s platform.
The Linhardt Research Labs.; The Shirley Ann Jackson, Ph.D. Center for Biotechnology and Interdisciplinary Studies (CBIS);
The Linhardt Research Labs Online Collection; Rensselaer Polytechnic Institute, Troy, NY; Advanced Functional Materials; https://harc.rpi.edu/;