Show simple item record

dc.contributor.authorDimitrievska, Sashka
dc.contributor.authorWang, Juan
dc.contributor.authorLin, Tylee
dc.contributor.authorWeyers, Amanda
dc.contributor.authorBai, Hualong
dc.contributor.authorQin, Lingfeng
dc.contributor.authorLi, Guangxin
dc.contributor.authorCai, Chao
dc.contributor.authorKypson, Alan
dc.contributor.authorKristofik, Nina
dc.contributor.authorGard, Ashley
dc.contributor.authorSundaram, Sumati
dc.contributor.authorYamamoto, Kota
dc.contributor.authorWu, Wei
dc.contributor.authorZhao, Liping
dc.contributor.authorKural, Mehmet H.
dc.contributor.authorYuan, Yifan
dc.contributor.authorMadri, Joseph
dc.contributor.authorKyriakides, Themis R.
dc.contributor.authorLinhardt, Robert J.
dc.contributor.authorNiklason, Laura E.
dc.identifier.citationGlycocalyx-like hydrogel coatings for small diameter vascular grafts, S. Dimitrievska, J. Wang, T. Lin, A. Weyers, H. Bai, L. Qin, G. Li, C. Cai, A. Kypson, N. Kristofik, A. Gard, S. Sundaram, K. Yamamoto, W. Wu, L. Zhao, M. H. Kural, Y. Yuan, J. Madri, T. R. Kyriakides, R. J. Linhardt, L. E. Niklason, Advanced Functional Matererials, 30, 1908963, 2020.
dc.descriptionAdvanced Functional Matererials, 30, 1908963
dc.descriptionNote : if this item contains full text it may be a preprint, author manuscript, or a Gold OA copy that permits redistribution with a license such as CC BY. The final version is available through the publisher’s platform.
dc.description.abstractNovel biological vascular conduits, such as decellularized tissue engineered vascular grafts (TEVGs) are hindered by high thrombogenicity. To mimic the antithrombogenic surface of native vessels with a continuous glycosaminoglycan layer that is present on endothelial cells (ECs), a hyaluronic acid (HA) modified surface is established, to effectively shield blood platelets from collagen-triggered activation. Using the amine groups present on 4 mm diameter decellularized TEVGs, a continuous HA hydrogel coating is built via a bifunctional thiol-reactive cross-linker, thereby avoiding nonspecific collagen matrix cross-linking. The HA hydrogel layer recreates a luminal wall, “hiding” exposed collagen from the bloodstream. In vitro blood tests show that adhered platelets, fibrinogen absorption, and fibrin formation on HA-coated decellularized TEVGs are significantly lower than on uncoated decellularized TEVGs. The HA surface also inhibits macrophage adhesion in vitro. HA-coated decellularized syngeneic rat aortae (≈1.5 mm diameter), and TEVGs in rat and canine models, respectively, are protected from aggressive thrombus formation, and preserve normal blood flow. Re-endothelialization is also observed. HA-coated TEVGs may be an off-the-shelf small-diameter vascular graft with dual benefits: antithrombogenic protection and promotion of endothelium.
dc.description.sponsorshipNational Institutes of Health
dc.relation.ispartofThe Linhardt Research Labs Online Collection
dc.relation.ispartofRensselaer Polytechnic Institute, Troy, NY
dc.relation.ispartofAdvanced Functional Materials
dc.subjectChemistry and chemical biology
dc.subjectChemical and biological engineering
dc.subjectBiomedical engineering
dc.titleGlycocalyx-like hydrogel coatings for small diameter vascular grafts
dc.rights.holderIn Copyright : this Item is protected by copyright and/or related rights. You are free to use this Item in any way that is permitted by the copyright and related rights legislation that applies to your use. For other uses you need to obtain permission from the rights-holder(s).
dc.relation.departmentThe Linhardt Research Labs.
dc.relation.departmentThe Shirley Ann Jackson, Ph.D. Center for Biotechnology and Interdisciplinary Studies (CBIS)

Files in this item


There are no files associated with this item.

This item appears in the following Collection(s)

Show simple item record