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dc.contributor.authorZhu, Kai
dc.contributor.authorMao, Guizhu
dc.contributor.authorWu, Dongmei
dc.contributor.authorYu, Chengxiao
dc.contributor.authorCheng, Huan
dc.contributor.authorXiao, Hang
dc.contributor.authorYe, Xingqian
dc.contributor.authorLinhardt, Robert J.
dc.contributor.authorOrfila, Caroline
dc.contributor.authorChen, Shiguo
dc.date2020
dc.date.accessioned2022-06-27T15:40:48Z
dc.date.available2022-06-27T15:40:48Z
dc.date.issued2020-08-12
dc.identifier.citationHighly branched RG-I domain enrichment are indispensable for pectin mitigating against high-fat diet-induced obesity, K. Zhu, G. Mao, D. Wu, C. Yu, H. Xiao, X. Ye, R. J. Linhardt, C. Orfila, S. Chen, Journal of Agriculture and Food Chemistry, 68, 8688-8701, 2020.
dc.identifier.issn15205118
dc.identifier.issn218561
dc.identifier.urihttps://doi.org/10.1021/acs.jafc.0c02654
dc.identifier.urihttps://hdl.handle.net/20.500.13015/5486
dc.descriptionJournal of Agriculture and Food Chemistry, 68, 8688-8701
dc.descriptionNote : if this item contains full text it may be a preprint, author manuscript, or a Gold OA copy that permits redistribution with a license such as CC BY. The final version is available through the publisher’s platform.
dc.description.abstractObesity is associated with gut microbiome dysbiosis. Our previous research has shown that highly branched rhamnogalacturonan type I (RG-I)-enriched pectin (WRP, 531.5 kDa, 70.44% RG-I, Rha/(Gal + Ara) = 20) and its oligosaccharide with less branched RG-I [DWRP, 12.1 kDa, 50.29% RG-I, Rha/(Gal + Ara) = 6] are potential prebiotics. The present study is conducted to uncover the impact of the content, molecular size, and branch degrees of RG-I on the inhibiting effect of high-fat diet (HFD)-induced obesity. The commercial pectin (CP, 496.2 kDa, 35.77% RG-I, Rha/(Gal + Ara) = 6), WRP, and DWRP were orally administered to HFD-fed C57BL/6J mice (100 mg kg–1 d–1) to determine their individual effects on obesity. WRP significantly prevented bodyweight gain, insulin resistance, and inflammatory responses in HFD-fed mice. No obvious anti-obesity effect was observed in either CP or DWRP supplementation. A mechanistic study revealed that CP and DWRP could not enhance the diversity of gut microbiota, while WRP treatment positively modulated the gut microbiota of obese mice by increasing the abundance of Butyrivibrio, Roseburia, Barnesiella, Flavonifractor, Acetivibrio, and Clostridium cluster IV. Furthermore, WRP significantly promoted browning of white adipose tissues in HFD-fed mice, while CP and DWRP did not. WRP can attenuate the HFD-induced obesity by modulation of gut microbiota and lipid metabolism. Highly branched RG-I domain enrichment is essential for pectin mitigating against the HFD-induced obesity.
dc.description.sponsorshipNational Natural Science Foundation of China
dc.description.urihttps://login.libproxy.rpi.edu/login?url=https://doi.org/10.1021/acs.jafc.0c02654
dc.languageen_US
dc.language.isoENG
dc.relation.ispartofThe Linhardt Research Labs Online Collection
dc.relation.ispartofRensselaer Polytechnic Institute, Troy, NY
dc.relation.ispartofJournal of Agricultural and Food Chemistry
dc.relation.urihttps://harc.rpi.edu/
dc.subjectBiology
dc.subjectChemistry and chemical biology
dc.subjectChemical and biological engineering
dc.subjectBiomedical engineering
dc.titleHighly branched RG-I domain enrichment are indispensable for pectin mitigating against high-fat diet-induced obesity
dc.typeArticle
dcterms.accessRightshttps://login.libproxy.rpi.edu/login?url=https://doi.org/10.1021/acs.jafc.0c02654
dcterms.isPartOfJournal
dcterms.isVersionOfhttps://doi.org/10.1021/acs.jafc.0c02654
dc.rights.holderIn Copyright : this Item is protected by copyright and/or related rights. You are free to use this Item in any way that is permitted by the copyright and related rights legislation that applies to your use. For other uses you need to obtain permission from the rights-holder(s). https://rightsstatements.org/page/InC/1.0/
dc.creator.identifierhttps://orcid.org/0000-0003-2219-5833
dc.relation.departmentThe Linhardt Research Labs.
dc.relation.departmentThe Shirley Ann Jackson, Ph.D. Center for Biotechnology and Interdisciplinary Studies (CBIS)
rpi.description.pages8688-8701
rpi.description.volume68


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