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dc.contributor.authorWen, Yan
dc.contributor.authorWen, Peng
dc.contributor.authorHu, Teng Gen
dc.contributor.authorLinhardt, Robert J.
dc.contributor.authorZong, Min Hua
dc.contributor.authorWu, Hong
dc.contributor.authorChen, Zhi Yi
dc.date2020
dc.date.accessioned2022-06-27T15:42:23Z
dc.date.available2022-06-27T15:42:23Z
dc.date.issued2020-04-15
dc.identifier.citationEncapsulation of phycocyanin by prebiotics and polysaccharides-based electrospun fibers and improved colon cancer prevention effects, Y. Wen, P. Wen, T.-G. Hu, R. J. Linhardt, M.-H. Zong , H. Wu, Z.-Y. Chen, International Journal of Biological Macromolecules 149, 672–681, 2020.
dc.identifier.issn18790003
dc.identifier.issn1418130
dc.identifier.urihttps://doi.org/10.1016/j.ijbiomac.2020.01.189
dc.identifier.urihttps://hdl.handle.net/20.500.13015/5512
dc.descriptionInternational Journal of Biological Macromolecules 149, 672–681
dc.descriptionNote : if this item contains full text it may be a preprint, author manuscript, or a Gold OA copy that permits redistribution with a license such as CC BY. The final version is available through the publisher’s platform.
dc.description.abstractTo preserve bioactivity and achieve colon targeted release of phycocyanin (PC), the polysaccharides-based electrospun fiber mat (EFM) containing PC and prebiotics was prepared and characterized. In vitro release tests confirmed the colon targeting behavior of PC, in particular, faster release of PC was achieved due to the addition of prebiotics. Ritger-Peppas model confirmed that the release of PC in simulated colon fluids follows a mechanism of anomalous transport (non-Fickian). CCK-8 results showed that the combination of PC and prebiotics exerted a significant anti-proliferative effect on HCT116 cells with an IC50 values of 22.31, 17.12 and 11.63 mg/mL after 24, 48, and 72 h, respectively. Furthermore, the cell cycle and apoptosis analysis revealed that the inhibition activity on HCT116 cells was caused by arresting cell cycle at G0/G1 phase that is relevant to the inhibition of cyclin D1 and CDK4 and the up-regulation of p21 expression, and inducing cell apoptosis by mediating the mitochondrial pathway as well, in which the decrease of Bcl-2/Bax, activation of caspase 3 and release of cytochrome c were included. This study suggests that the PC-loaded EFM with GOS holds a great potential as an effective formulation for colon cancer prevention.
dc.description.sponsorshipNational Natural Science Foundation of China
dc.description.urihttps://login.libproxy.rpi.edu/login?url=https://doi.org/10.1016/j.ijbiomac.2020.01.189
dc.languageen_US
dc.language.isoENG
dc.relation.ispartofThe Linhardt Research Labs Online Collection
dc.relation.ispartofRensselaer Polytechnic Institute, Troy, NY
dc.relation.ispartofInternational Journal of Biological Macromolecules
dc.relation.urihttps://harc.rpi.edu/
dc.subjectBiology
dc.subjectChemistry and chemical biology
dc.subjectChemical and biological engineering
dc.subjectBiomedical engineering
dc.titleEncapsulation of phycocyanin by prebiotics and polysaccharides-based electrospun fibers and improved colon cancer prevention effects
dc.typeArticle
dcterms.accessRightshttps://login.libproxy.rpi.edu/login?url=https://doi.org/10.1016/j.ijbiomac.2020.01.189
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dcterms.isVersionOfhttps://doi.org/10.1016/j.ijbiomac.2020.01.189
dc.rights.holderIn Copyright : this Item is protected by copyright and/or related rights. You are free to use this Item in any way that is permitted by the copyright and related rights legislation that applies to your use. For other uses you need to obtain permission from the rights-holder(s). https://rightsstatements.org/page/InC/1.0/
dc.creator.identifierhttps://orcid.org/0000-0003-2219-5833
dc.relation.departmentThe Linhardt Research Labs.
dc.relation.departmentThe Shirley Ann Jackson, Ph.D. Center for Biotechnology and Interdisciplinary Studies (CBIS)
rpi.description.pages672-681
rpi.description.volume149


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