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dc.contributor.authorCheng, Leiyu
dc.contributor.authorZhang, Han
dc.contributor.authorLiang, Hao
dc.contributor.authorSun, Xinxiao
dc.contributor.authorShen, Xiaolin
dc.contributor.authorWang, Jia
dc.contributor.authorWang, Wenya
dc.contributor.authorYuan, Qipeng
dc.contributor.authorRi, Hyon Il
dc.contributor.authorKim, Tae Mun
dc.contributor.authorKang, Myong Su
dc.contributor.authorLinhardt, Robert J.
dc.identifier.citationEnzymatic Bioconversion of Cycloastragenol-6-O-β-D-glucoside into Cycloastragenol by a Novel Recombinant β-Glucosidase from Phycicoccus sp. Soil748, L. Cheng, H. Zhang, H. Liang, X. Sun, X. Shen, J. Wang, W. Wang, Q. Yuan, H.-I. Ri, T.-M. Kim, M.-S. Kang, R. J. Linhardt, Process Biochemistry, 90, 81–88, 2019.
dc.descriptionProcess Biochemistry, 90, 81–88
dc.descriptionNote : if this item contains full text it may be a preprint, author manuscript, or a Gold OA copy that permits redistribution with a license such as CC BY. The final version is available through the publisher’s platform.
dc.description.abstractCycloastragenol (CA), the genuine sapogenin of astragaloside from Astragalus membranaceus, exhibits diverse pharmaceutical activities. Recently, the efficient production of CA has received considerable attention due to rapidly increasing market demands. In this study, enzyme mining was conducted, based on skeleton and glycosyl similarity, to explore an efficient β-glucosidase for CA preparation. A novel β-glucosidase from Phycicoccus sp. Soil748 (Bgps) was discovered, possessing the efficient conversion rate for cycloastragenol-6-O-β-D-glucoside (CMG) into CA. The optimum temperature and pH value of Bgps were determined as 45 °C and 7.0. The results of kinetic analysis suggested that Bgps catalyzed deglycosylation of CMG more efficiently than other substrates. Furthermore, the optimal substrate concentration of Bgps was up to 80 mg/mL with the conversion rate as 99.2%, suggesting its potential application in CA industrial production by biotransformation.
dc.description.sponsorshipNational Key Research and Development Program of China Stem Cell and Translational Research
dc.relation.ispartofThe Linhardt Research Labs Online Collection
dc.relation.ispartofRensselaer Polytechnic Institute, Troy, NY
dc.relation.ispartofProcess Biochemistry
dc.subjectChemistry and chemical biology
dc.subjectChemical and biological engineering
dc.subjectBiomedical engineering
dc.titleEnzymatic Bioconversion of Cycloastragenol-6-O-β-D-glucoside into Cycloastragenol by a Novel Recombinant β-Glucosidase from Phycicoccus sp. Soil748
dc.rights.holderIn Copyright : this Item is protected by copyright and/or related rights. You are free to use this Item in any way that is permitted by the copyright and related rights legislation that applies to your use. For other uses you need to obtain permission from the rights-holder(s).
dc.relation.departmentThe Linhardt Research Labs.
dc.relation.departmentThe Shirley Ann Jackson, Ph.D. Center for Biotechnology and Interdisciplinary Studies (CBIS)

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