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dc.contributor.authorYan, Lufeng
dc.contributor.authorWang, D.
dc.contributor.authorZhu, Mengshan
dc.contributor.authorYu, Yanlei
dc.contributor.authorZhang, Fuming
dc.contributor.authorYe, Xingqian
dc.contributor.authorLinhardt, Robert J.
dc.contributor.authorChen, Shiguo
dc.date2019
dc.date.accessioned2022-06-27T15:46:20Z
dc.date.available2022-06-27T15:46:20Z
dc.date.issued2019-10-15
dc.identifier.citationHighly purified fucosylated chondroitin sulfate oligomers with selective intrinsic factor Xase complex inhibition, L. Yan, D. Wang, M. Zhu, Y. Yu, F. Zhang, X. Ye, R. J. Linhardt, S. Chen, Carbohydrate Polymers,222, 115025, 2019.
dc.identifier.issn1448617
dc.identifier.urihttps://doi.org/10.1016/j.carbpol.2019.115025
dc.identifier.urihttps://hdl.handle.net/20.500.13015/5534
dc.descriptionCarbohydrate Polymers,222, 115025
dc.descriptionNote : if this item contains full text it may be a preprint, author manuscript, or a Gold OA copy that permits redistribution with a license such as CC BY. The final version is available through the publisher’s platform.
dc.description.abstractFucosylated chondroitin sulfate (FCS) oligosaccharides of specific molecular weight have shown potent anticoagulant activities with selectivity towards intrinsic factor Xase complex. However, the preparation of FCS oligosaccharides by traditional methods requires multiple purification steps consuming large amounts of time and significant resources. The current study focuses on developing a method for the rapid preparation of FCS oligomers from sea cucumber Pearsonothuria graeffei having 6-18 saccharide residues. The key steps controlling molecular weight (Mw) and purity of these FCS oligomers were evaluated. Structural analysis showed the resulting FCS oligomers were primarily l-Fuc3,4diS-α1,3-d-GlcA-β1,3-(d-GalNAc4,6diS-β1,4-[l-Fuc3,4diS-α1,3-]d-GlcA-β1,3-)nd-anTal-ol4,6diS (n = 1˜5) accompanied by partial de-fucosylation and/or de-sulfation. In vitro and in vivo experiments demonstrate that these FCS oligomers selectively inhibit intrinsic factor Xase complex and exhibit remarkable antithrombotic activity without hemorrhagic and hypotension side effects. This method is suitable for large-scale preparation of FCS oligosaccharides as clinical anticoagulants.
dc.description.sponsorshipRensselaer Polytechnic Institute
dc.description.urihttps://login.libproxy.rpi.edu/login?url=https://doi.org/10.1016/j.carbpol.2019.115025
dc.languageen_US
dc.language.isoENG
dc.relation.ispartofThe Linhardt Research Labs Online Collection
dc.relation.ispartofRensselaer Polytechnic Institute, Troy, NY
dc.relation.ispartofCarbohydrate Polymers
dc.relation.urihttps://harc.rpi.edu/
dc.subjectBiology
dc.subjectChemistry and chemical biology
dc.subjectChemical and biological engineering
dc.subjectBiomedical engineering
dc.titleHighly purified fucosylated chondroitin sulfate oligomers with selective intrinsic factor Xase complex inhibition
dc.typeArticle
dcterms.accessRightshttps://login.libproxy.rpi.edu/login?url=https://doi.org/10.1016/j.carbpol.2019.115025
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dcterms.isVersionOfhttps://doi.org/10.1016/j.carbpol.2019.115025
dc.rights.holderIn Copyright : this Item is protected by copyright and/or related rights. You are free to use this Item in any way that is permitted by the copyright and related rights legislation that applies to your use. For other uses you need to obtain permission from the rights-holder(s). https://rightsstatements.org/page/InC/1.0/
dc.creator.identifierhttps://orcid.org/0000-0003-2219-5833
dc.relation.departmentThe Linhardt Research Labs.
dc.relation.departmentThe Shirley Ann Jackson, Ph.D. Center for Biotechnology and Interdisciplinary Studies (CBIS)
rpi.description.volume222


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