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dc.contributor.authorKim, S.Y.
dc.contributor.authorKundu, J.
dc.contributor.authorWilliams, A.
dc.contributor.authorYandulskaya, A.S.
dc.contributor.authorMonaghan, J.R.
dc.contributor.authorCarrier, R.L.
dc.contributor.authorLinhardt, Robert J.
dc.date2019
dc.date.accessioned2022-06-27T15:47:40Z
dc.date.available2022-06-27T15:47:40Z
dc.date.issued2019
dc.identifier.citationGlycosaminoglycans compositional analysis of urodele axolotl (Ambystoma mexicanum) and porcine retina, S. Y. Kim, J. Kundu, A. Williams, A. S. Yandulskaya, J. R. Monaghan, R. L. Carrier, R. J. Linhardt, Glycoconjugate Journal, 36, 165–174, 2019.
dc.identifier.urihttps://doi.org/10.1007/s10719-019-09863-5
dc.identifier.urihttps://hdl.handle.net/20.500.13015/5549
dc.descriptionGlycoconjugate Journal, 36, 165–174
dc.descriptionNote : if this item contains full text it may be a preprint, author manuscript, or a Gold OA copy that permits redistribution with a license such as CC BY. The final version is available through the publisher’s platform.
dc.description.abstractRetinal degenerative diseases, such as age-related macular degeneration (AMD) and retinitis pigmentosa (RP), are major causes of blindness worldwide. Humans cannot regenerate retina, however, axolotl (Ambystoma mexicanum), a laboratory-bred salamander, can regenerate retinal tissue throughout adulthood. Classic signaling pathways, including fibroblast growth factor (FGF), are involved in axolotl regeneration. Glycosaminoglycan (GAG) interaction with FGF is required for signal transduction in this pathway. GAGs are anionic polysaccharides in extracellular matrix (ECM) that have been implicated in limb and lens regeneration of amphibians, however, GAGs have not been investigated in the context of retinal regeneration. GAG composition is characterized native and decellularized axolotl and porcine retina using liquid chromatography mass spectrometry. Pig was used as a mammalian vertebrate model without the ability to regenerate retina. Chondroitin sulfate (CS) was the main retinal GAG, followed by heparan sulfate (HS), hyaluronic acid, and keratan sulfate in both native and decellularized axolotl and porcine retina. Axolotl retina exhibited a distinctive GAG composition pattern in comparison with porcine retina, including a higher content of hyaluronic acid. In CS, higher levels of 4- and 6- O-sulfation were observed in axolotl retina. The HS composition was greater in decellularized tissues in both axolotl and porcine retina by 7.1% and 15.4%, respectively, and different sulfation patterns were detected in axolotl. Our findings suggest a distinctive GAG composition profile of the axolotl retina set foundation for role of GAGs in homeostatic and regenerative conditions of the axolotl retina and may further our understanding of retinal regenerative models.
dc.description.urihttps://login.libproxy.rpi.edu/login?url=https://doi.org/10.1007/s10719-019-09863-5
dc.languageen_US
dc.language.isoENG
dc.relation.ispartofThe Linhardt Research Labs Online Collection
dc.relation.ispartofRensselaer Polytechnic Institute, Troy, NY
dc.relation.urihttps://harc.rpi.edu/
dc.subjectBiology
dc.subjectChemistry and chemical biology
dc.subjectChemical and biological engineering
dc.subjectBiomedical engineering
dc.titleGlycosaminoglycans compositional analysis of urodele axolotl (Ambystoma mexicanum) and porcine retina
dc.typeArticle
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dcterms.isVersionOfhttps://doi.org/10.1007/s10719-019-09863-5
dc.rights.holderIn Copyright : this Item is protected by copyright and/or related rights. You are free to use this Item in any way that is permitted by the copyright and related rights legislation that applies to your use. For other uses you need to obtain permission from the rights-holder(s). https://rightsstatements.org/page/InC/1.0/
dc.creator.identifierhttps://orcid.org/0000-0003-2219-5833
dc.relation.departmentThe Linhardt Research Labs.
dc.relation.departmentThe Shirley Ann Jackson, Ph.D. Center for Biotechnology and Interdisciplinary Studies (CBIS)


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