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dc.contributor.authorHe, Zhe
dc.contributor.authorHan, Zehua
dc.contributor.authorKizer, Megan
dc.contributor.authorLinhardt, Robert J.
dc.contributor.authorWang, Xing
dc.contributor.authorSinyukov, Alexander M.
dc.contributor.authorWang, Jizhou
dc.contributor.authorDeckert, Volker
dc.contributor.authorSokolov, Alexei V.
dc.contributor.authorHu, Jonathan
dc.contributor.authorScully, Marlan O.
dc.identifier.citationTip-enhanced Raman imaging of single-stranded DNA with single base resolution, Z. He, Z. Han, M. Kizer, R. Linhardt, X. Wang, A. Sinyukov, J. Wang, V. Deckert, A. Sokolov, J. Hu, M. Scully, Journal of the American Chemical Society, 141, 753−757, 2019.
dc.descriptionJournal of the American Chemical Society, 141, 753−757
dc.descriptionNote : if this item contains full text it may be a preprint, author manuscript, or a Gold OA copy that permits redistribution with a license such as CC BY. The final version is available through the publisher’s platform.
dc.description.abstractTip-enhanced Raman scattering (TERS) is a promising optical and analytical technique for chemical imaging and sensing at single molecule resolution. In particular, TERS signals generated by a gap-mode configuration where a silver tip is coupled with a gold substrate can resolve a single-stranded DNA (ssDNA) molecule with a spatial resolution below 1 nm. To demonstrate the proof of subnanometer resolution, we show direct nucleic acid sequencing using TERS of a phage ssDNA (M13mp18). M13mp18 provides a known sequence and, through our deposition strategy, can be stretched (uncoiled) and attached to the substrate by its phosphate groups, while exposing its nucleobases to the tip. After deposition, we scan the silver tip along the ssDNA and collect TERS signals with a step of 0.5 nm, comparable to the bond length between two adjacent DNA bases. By demonstrating the real-time profiling of a ssDNA configuration and furthermore, with unique TERS signals of monomeric units of other biopolymers, we anticipate that this technique can be extended to the high-resolution imaging of various nanostructures as well as the direct sequencing of other important biopolymers including RNA, polysaccharides, and polypeptides.
dc.description.sponsorshipNational Science Foundation
dc.relation.ispartofThe Linhardt Research Labs Online Collection
dc.relation.ispartofRensselaer Polytechnic Institute, Troy, NY
dc.relation.ispartofJournal of the American Chemical Society
dc.subjectChemistry and chemical biology
dc.subjectChemical and biological engineering
dc.subjectBiomedical engineering
dc.titleTip-enhanced Raman imaging of single-stranded DNA with single base resolution
dc.rights.holderIn Copyright : this Item is protected by copyright and/or related rights. You are free to use this Item in any way that is permitted by the copyright and related rights legislation that applies to your use. For other uses you need to obtain permission from the rights-holder(s).
dc.relation.departmentThe Linhardt Research Labs.
dc.relation.departmentThe Shirley Ann Jackson, Ph.D. Center for Biotechnology and Interdisciplinary Studies (CBIS)

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