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    Bacteriophage T7 transcription system: an enabling tool in synthetic biology

    Author
    Wang, Wenya; Li, Yuwenbin; Wang, Yaqiong; Shi, Chen; Li, Chenmeng; Li, Qiang; Linhardt, Robert J.
    ORCID
    https://orcid.org/0000-0003-2219-5833
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    Other Contributors
    Date Issued
    2018-12-01
    Subject
    Biology; Chemistry and chemical biology; Chemical and biological engineering; Biomedical engineering
    Degree
    Terms of Use
    In Copyright : this Item is protected by copyright and/or related rights. You are free to use this Item in any way that is permitted by the copyright and related rights legislation that applies to your use. For other uses you need to obtain permission from the rights-holder(s). https://rightsstatements.org/page/InC/1.0/;
    Full Citation
    Bacteriophage T7 transcription system: an enabling tool in synthetic biology, W. Wang, Y. Li, Y. Wang, C. Shi, C. Li, Q. Li, R. J. Linhardt, Biotechnology Advances, 36, 2129–2137, 2018.
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    URI
    https://doi.org/10.1016/j.biotechadv.2018.10.001; https://hdl.handle.net/20.500.13015/5575
    Abstract
    Since its discovery in the 1970s, the T7 RNA polymerase (T7 RNAP) transcription system has been applied extensively as an effective tool in molecular biology because of its robust function in various hosts, including prokaryotic, eukaryotic and cell free systems. Recently, the T7 RNAP transcription system has emerged as a critical component for synthetic biology. The present paper summarizes the advances of the T7 RNAP transcription system in synthetic biology, including the recent progress of T7 RNAP structure and its cognate promoter and terminator and its application in cell free systems, logic gates and orthogonal genetic circuits.;
    Description
    Biotechnology Advances, 36, 2129–2137; Note : if this item contains full text it may be a preprint, author manuscript, or a Gold OA copy that permits redistribution with a license such as CC BY. The final version is available through the publisher’s platform.
    Department
    The Linhardt Research Labs.; The Shirley Ann Jackson, Ph.D. Center for Biotechnology and Interdisciplinary Studies (CBIS);
    Relationships
    The Linhardt Research Labs Online Collection; Rensselaer Polytechnic Institute, Troy, NY; Biotechnology Advances; https://harc.rpi.edu/;
    Access
    https://login.libproxy.rpi.edu/login?url=https://doi.org/10.1016/j.biotechadv.2018.10.001;
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