Author
Wang, Wenya; Li, Yuwenbin; Wang, Yaqiong; Shi, Chen; Li, Chenmeng; Li, Qiang; Linhardt, Robert J.
Other Contributors
Date Issued
2018-12-01
Subject
Biology; Chemistry and chemical biology; Chemical and biological engineering; Biomedical engineering
Degree
Terms of Use
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Full Citation
Bacteriophage T7 transcription system: an enabling tool in synthetic biology, W. Wang, Y. Li, Y. Wang, C. Shi, C. Li, Q. Li, R. J. Linhardt, Biotechnology Advances, 36, 2129–2137, 2018.
Abstract
Since its discovery in the 1970s, the T7 RNA polymerase (T7 RNAP) transcription system has been applied extensively as an effective tool in molecular biology because of its robust function in various hosts, including prokaryotic, eukaryotic and cell free systems. Recently, the T7 RNAP transcription system has emerged as a critical component for synthetic biology. The present paper summarizes the advances of the T7 RNAP transcription system in synthetic biology, including the recent progress of T7 RNAP structure and its cognate promoter and terminator and its application in cell free systems, logic gates and orthogonal genetic circuits.;
Description
Biotechnology Advances, 36, 2129–2137; Note : if this item contains full text it may be a preprint, author manuscript, or a Gold OA copy that permits redistribution with a license such as CC BY. The final version is available through the publisher’s platform.
Department
The Linhardt Research Labs.; The Shirley Ann Jackson, Ph.D. Center for Biotechnology and Interdisciplinary Studies (CBIS);
Relationships
The Linhardt Research Labs Online Collection; Rensselaer Polytechnic Institute, Troy, NY; Biotechnology Advances; https://harc.rpi.edu/;
Access
https://login.libproxy.rpi.edu/login?url=https://doi.org/10.1016/j.biotechadv.2018.10.001;
