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dc.contributor.authorKhan, S.
dc.contributor.authorSzabo, Z.
dc.contributor.authorLiu, J.
dc.contributor.authorKunnummal, B.
dc.contributor.authorHan, X.
dc.contributor.authorOuyang, Y.
dc.contributor.authorLinhardt, Robert J.
dc.contributor.authorXia, Q.
dc.identifier.citationOn-line CE-LIF-MS analysis of glycans labeled with teal fluorescence dye using electrokinetic sheath liquid pump-based nanospray ion source, S. Khan, Z. Szabo, J. Liu, B. Kunnummal, X. Han, Y. Ouyang, R. J. Linhardt, Q. Xia, Rapid Communications in Mass Spectrometry, 32, 882–888, 2018.
dc.descriptionRapid Communications in Mass Spectrometry, 32, 882–888
dc.descriptionNote : if this item contains full text it may be a preprint, author manuscript, or a Gold OA copy that permits redistribution with a license such as CC BY. The final version is available through the publisher’s platform.
dc.description.abstractRationale: N-linked glycan analysis of recombinant therapeutic proteins, such as monoclonal antibodies, Fc-fusion proteins, and antibody-drug conjugates, provides valuable information regarding protein therapeutics glycosylation profile. Both qualitative identification and quantitative analysis of N-linked glycans on recombinant therapeutic proteins are critical analytical tasks in the biopharma industry during the development of a biotherapeutic. Methods: Currently, such analyses are mainly carried out using capillary electrophoresis/laser-induced fluorescence (CE/LIF), liquid chromatography/fluorescence (LC/FLR), and liquid chromatography/fluorescence/mass spectrometry (LC/FLR/MS) technologies. N-linked glycans are first released from glycoproteins by enzymatic digestion, then labeled with fluorescence dyes for subsequent CE or LC separation, and LIF or MS detection. Here we present an on-line CE/LIF/MS N-glycan analysis workflow that incorporates the fluorescent Teal™ dye and an electrokinetic pump-based anospray sheath liquid capillary electrophoresis/mass spectrometry (CE/MS) ion source. Results: Electrophoresis running buffer systems using ammonium acetate and ammonium hydroxide were developed for the negative ion mode CE/MS analysis of fluorescence-labeled N-linked glycans. Results show that on-line CE/LIF/MS analysis can be readily achieved using this versatile CE/MS ion source on common CE/MS instrument platforms. Conclusions: This on-line CE/LIF/MS method using Teal™ fluorescent dye and electrokinetic pump-based nanospray sheath liquid CE/MS coupling technology holds promise for on-line quantitation and identification of N-linked glycans on recombinant therapeutic proteins.
dc.relation.ispartofThe Linhardt Research Labs Online Collection
dc.relation.ispartofRensselaer Polytechnic Institute, Troy, NY
dc.subjectChemistry and chemical biology
dc.subjectChemical and biological engineering
dc.subjectBiomedical engineering
dc.titleOn-line CE-LIF-MS analysis of glycans labeled with teal fluorescence dye using electrokinetic sheath liquid pump-based nanospray ion source
dc.rights.holderIn Copyright : this Item is protected by copyright and/or related rights. You are free to use this Item in any way that is permitted by the copyright and related rights legislation that applies to your use. For other uses you need to obtain permission from the rights-holder(s).
dc.relation.departmentThe Linhardt Research Labs.
dc.relation.departmentThe Shirley Ann Jackson, Ph.D. Center for Biotechnology and Interdisciplinary Studies (CBIS)

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