Biology; Chemistry and chemical biology; Chemical and biological engineering; Biomedical engineering
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Purification and structural elucidation of a water-soluble polysaccharide from the fruiting bodies of the Grifola frondosa. A. Zhang, J. Deng, S. Yu, F. Zhang, R. J. Linhardt, P. Sun, International Journal of Biological Macromolecules, 115, 221–226, 2018.
Grifola frondosa is a polypore mushroom, which has been used for many centuries by traditional Chinese and Japanese herbalists as a medicinal mushroom. A water-soluble polysaccharide (code as GFP30-2-a, molecular weight: 2.04×106Da) was isolated from the fruiting bodies of G. frondosa by hot water extraction, ethanol precipitation and chromatography. Its structure was elucidated from its monosaccharide composition, methylation analysis, together with 1D NMR (1H NMR and 13C NMR) and 2D NMR (COSY, TOCSY, HSQC, HMBC and NOESY spectra). GFP30-2-a consists of D-Glc and D-Gal in the molar ratio of 1:0.098 and the structure of the repeating units was identified to be β‑d‑Glcp‑(1→[4)‑α‑d‑Glcp‑(1→4)‑α‑d‑Glcp‑(1]m→4)‑α‑d‑Glcp.;
International Journal of Biological Macromolecules, 115, 221–226; Note : if this item contains full text it may be a preprint, author manuscript, or a Gold OA copy that permits redistribution with a license such as CC BY. The final version is available through the publisher’s platform.
The Linhardt Research Labs.; The Shirley Ann Jackson, Ph.D. Center for Biotechnology and Interdisciplinary Studies (CBIS);
The Linhardt Research Labs Online Collection; Rensselaer Polytechnic Institute, Troy, NY; International Journal of Biological Macromolecules; https://harc.rpi.edu/;