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    Purification and structural elucidation of a water-soluble polysaccharide from the fruiting bodies of the Grifola frondosa

    Author
    Zhang, Anqiang; Deng, Jiaying; Yu, Shuying; Zhang, Fuming; Linhardt, Robert J.; Sun, Peilong
    ORCID
    https://orcid.org/0000-0003-2219-5833
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    Other Contributors
    Date Issued
    2018-08-01
    Subject
    Biology; Chemistry and chemical biology; Chemical and biological engineering; Biomedical engineering
    Degree
    Terms of Use
    In Copyright : this Item is protected by copyright and/or related rights. You are free to use this Item in any way that is permitted by the copyright and related rights legislation that applies to your use. For other uses you need to obtain permission from the rights-holder(s). https://rightsstatements.org/page/InC/1.0/;
    Full Citation
    Purification and structural elucidation of a water-soluble polysaccharide from the fruiting bodies of the Grifola frondosa. A. Zhang, J. Deng, S. Yu, F. Zhang, R. J. Linhardt, P. Sun, International Journal of Biological Macromolecules, 115, 221–226, 2018.
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    URI
    https://doi.org/10.1016/j.ijbiomac.2018.04.061; https://hdl.handle.net/20.500.13015/5593
    Abstract
    Grifola frondosa is a polypore mushroom, which has been used for many centuries by traditional Chinese and Japanese herbalists as a medicinal mushroom. A water-soluble polysaccharide (code as GFP30-2-a, molecular weight: 2.04×106Da) was isolated from the fruiting bodies of G. frondosa by hot water extraction, ethanol precipitation and chromatography. Its structure was elucidated from its monosaccharide composition, methylation analysis, together with 1D NMR (1H NMR and 13C NMR) and 2D NMR (COSY, TOCSY, HSQC, HMBC and NOESY spectra). GFP30-2-a consists of D-Glc and D-Gal in the molar ratio of 1:0.098 and the structure of the repeating units was identified to be β‑d‑Glcp‑(1→[4)‑α‑d‑Glcp‑(1→4)‑α‑d‑Glcp‑(1]m→4)‑α‑d‑Glcp.;
    Description
    International Journal of Biological Macromolecules, 115, 221–226; Note : if this item contains full text it may be a preprint, author manuscript, or a Gold OA copy that permits redistribution with a license such as CC BY. The final version is available through the publisher’s platform.
    Department
    The Linhardt Research Labs.; The Shirley Ann Jackson, Ph.D. Center for Biotechnology and Interdisciplinary Studies (CBIS);
    Relationships
    The Linhardt Research Labs Online Collection; Rensselaer Polytechnic Institute, Troy, NY; International Journal of Biological Macromolecules; https://harc.rpi.edu/;
    Access
    https://login.libproxy.rpi.edu/login?url=https://doi.org/10.1016/j.ijbiomac.2018.04.061;
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